HLA-DM Antibody (MaP.DM1): sc-32248
- HLA-DM Antibody (MaP.DM1) is a mouse monoclonal IgG1 κ HLA-DM antibody, cited in 5 publications, provided at 200 µg/ml
- raised against MHC class II-enriched compartments of human B cells
- HLA-DM Antibody (MaP.DM1) is recommended for detection of HLA-DM of human origin by IF and FCM
- Anti-HLA-DM Antibody (MaP.DM1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HLA-DM Antibody (MaP.DM1). These reagents are now offered in bundles with HLA-DM Antibody (MaP.DM1) (see ordering information below).
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HLA-DM Antibody (MaP.DM1) is a mouse monoclonal IgG1 kappa light chain antibody that detects HLA-DM protein of human origin by immunofluorescence (IF) and flow cytometry (FCM). Anti-HLA-DM antibody (MaP.DM1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HLA-DM plays a crucial role in the immune response by facilitating the loading of peptides onto major histocompatibility complex (MHC) class II molecules, which are essential for the activation of CD4+ T cells. Specifically, HLA-DM assists in the removal of class II-associated invariant chain peptides (CLIP) from the MHC class II binding groove, allowing for the binding of exogenous peptides that are vital for T cell recognition. This process is particularly important in the context of autoimmune diseases, as variations in the HLA-DM gene have been linked to susceptibility to conditions such as rheumatoid arthritis. Understanding HLA-DM function and interactions within the immune system provides valuable insights into immune regulation mechanisms and potential therapeutic targets for autoimmune disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HLA-DM Antibody (MaP.DM1) References:
- HLA-DMA and HLA-DMB alleles in German patients with type 1 diabetes mellitus. | Siegmund, T., et al. 1999. Tissue Antigens. 54: 291-4. PMID: 10519369
- The association of HLA-DM genes with rheumatoid arthritis in Eastern France. | Toussirot, E., et al. 2000. Hum Immunol. 61: 303-8. PMID: 10689120
- Functional HLA-DM on the surface of B cells and immature dendritic cells. | Arndt, SO., et al. 2000. EMBO J. 19: 1241-51. PMID: 10716924
- Functional characterization of a lysosomal sorting motif in the cytoplasmic tail of HLA-DObeta. | Brunet, A., et al. 2000. J Biol Chem. 275: 37062-71. PMID: 10964920
- Determination of the HLA-DM interaction site on HLA-DR molecules. | Doebele, RC., et al. 2000. Immunity. 13: 517-27. PMID: 11070170
- Modulation of monocyte activation in patients with rheumatoid arthritis by leukapheresis therapy. | Hahn, G., et al. 1993. J Clin Invest. 91: 862-70. PMID: 8450066
- A role for HLA-DO as a co-chaperone of HLA-DM in peptide loading of MHC class II molecules. | Kropshofer, H., et al. 1998. EMBO J. 17: 2971-81. PMID: 9606180
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-DM Antibody (MaP.DM1) | sc-32248 | 200 µg/ml | $322.00 | |||
HLA-DM Antibody (MaP.DM1): m-IgG Fc BP-HRP Bundle | sc-526670 | 200 µg Ab; 10 µg BP | $361.00 | |||
HLA-DM Antibody (MaP.DM1): m-IgG1 BP-HRP Bundle | sc-532043 | 200 µg Ab; 20 µg BP | $361.00 | |||
HLA-DM Antibody (MaP.DM1) AC | sc-32248 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HLA-DM Antibody (MaP.DM1) HRP | sc-32248 HRP | 200 µg/ml | $322.00 | |||
HLA-DM Antibody (MaP.DM1) FITC | sc-32248 FITC | 200 µg/ml | $336.00 | |||
HLA-DM Antibody (MaP.DM1) PE | sc-32248 PE | 200 µg/ml | $349.00 | |||
HLA-DM Antibody (MaP.DM1) Alexa Fluor® 488 | sc-32248 AF488 | 200 µg/ml | $364.00 | |||
HLA-DM Antibody (MaP.DM1) Alexa Fluor® 546 | sc-32248 AF546 | 200 µg/ml | $364.00 | |||
HLA-DM Antibody (MaP.DM1) Alexa Fluor® 594 | sc-32248 AF594 | 200 µg/ml | $364.00 | |||
HLA-DM Antibody (MaP.DM1) Alexa Fluor® 647 | sc-32248 AF647 | 200 µg/ml | $364.00 | |||
HLA-DM Antibody (MaP.DM1) Alexa Fluor® 680 | sc-32248 AF680 | 200 µg/ml | $364.00 | |||
HLA-DM Antibody (MaP.DM1) Alexa Fluor® 790 | sc-32248 AF790 | 200 µg/ml | $364.00 |