HIVEP3 CRISPR/Cas9 KO Plasmid (m): sc-421334
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HIVEP3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HIVEP3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIVEP3 CRISPR/Cas9 KO Plasmid (m) | sc-421334 | 20 µg | $397.00 |
Overview
Hivep3 encodes HIVEP3, a large zinc finger transcription factor that binds κB-like DNA elements and modulates stimulus-responsive gene expression programs. HIVEP3 is linked to regulation of immune and inflammatory signaling networks, including transcriptional outputs downstream of NF-κB and related pathways that coordinate cytokine production, lymphocyte activation, and cellular stress responses. In mouse systems, altered HIVEP3 activity has been associated with dysregulated immune homeostasis and inflammatory phenotypes, supporting its relevance for mechanistic studies of autoimmunity and tissue inflammation. Because it integrates sequence-specific DNA binding with broad transcriptional control, Hivep3 is also used as a node for studying gene regulatory circuitry and cell-state transitions.
HIVEP3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hivep3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hivep3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hivep3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HIVEP3 protein expression.
This CRISPR knockout system enables efficient generation of Hivep3-deficient cell models for investigation of HIVEP3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hivep3 exon(s) critical for HIVEP3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hivep3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HIVEP3 CRISPR/Cas9 KO Plasmid (m) and HIVEP3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hivep3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HIVEP3 HDR Plasmid (m) and HIVEP3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hivep3 homology arms to support homology-directed repair at defined Hivep3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.