Histone H1X Double Nickase Plasmid (h): sc-411232-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone H1X Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Histone H1X Double Nickase Plasmid (h) and Histone H1X Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting H1FX. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histone H1X Antibody (E-5): sc-514856
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone H1X Double Nickase Plasmid (h) | sc-411232-NIC | 20 µg | $410.00 | |||
Histone H1X Double Nickase Plasmid (h2) | sc-411232-NIC-2 | 20 µg | $410.00 |
H1FX encodes histone H1X, a linker histone variant that binds nucleosomal DNA and contributes to higher-order chromatin compaction and genome organization. By modulating nucleosome spacing and chromatin accessibility, H1X influences transcriptional regulation, DNA replication timing, and DNA damage responses within epigenetic control pathways. Altered abundance or redistribution of H1 family proteins can reshape chromatin states that affect cell identity programs and stress-adaptive gene expression. Consequently, H1FX is frequently studied in the context of chromatin dysregulation relevant to cancer biology, developmental processes, and genome stability phenotypes.
Histone H1X Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the H1FX locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within H1FX. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt H1FX function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of H1FX-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.