Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Histone H1X CRISPR Activation Plasmid (h): sc-411232-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone H1X CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Histone H1X CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Histone H1X CRISPR Activation Plasmid (h) and Histone H1X CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the H1FX transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histone H1X Antibody (E-5): sc-514856
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone H1X CRISPR Activation Plasmid (h) | sc-411232-ACT | 20 µg | $397.00 | |||
Histone H1X CRISPR Activation Plasmid (h2) | sc-411232-ACT-2 | 20 µg | $397.00 |
H1FX encodes histone H1X, a linker histone variant that binds nucleosomal DNA and contributes to higher-order chromatin compaction and genome organization. By modulating nucleosome spacing and chromatin accessibility, histone H1X influences transcriptional regulation, DNA replication timing, and DNA damage responses through chromatin remodeling pathways. Altered H1FX expression or histone H1 variant balance has been associated with epigenetic dysregulation that can impact cell-state transitions, proliferation programs, and genome stability. These features make H1FX a useful target for studying chromatin architecture, transcriptional networks, and disease-relevant epigenetic mechanisms in human cells.
Histone H1X CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous H1FX expression without altering the underlying DNA sequence.
Histone H1X CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the H1FX locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the H1FX transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Histone H1X expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native H1FX locus and enabling the study of Histone H1X-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Histone H1X pathway restoration in tumor cells with silenced or reduced H1FX expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.