Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Histone H1 Double Nickase Plasmid (h): sc-404845-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone H1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Histone H1 Double Nickase Plasmid (h) and Histone H1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HIST1H1B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histone H1 Antibody (H-2): sc-393358
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone H1 Double Nickase Plasmid (h) | sc-404845-NIC | 20 µg | $410.00 | |||
Histone H1 Double Nickase Plasmid (h2) | sc-404845-NIC-2 | 20 µg | $410.00 |
HIST1H1B encodes the canonical linker histone H1.5 (Histone H1), a key chromatin architectural protein that binds nucleosomal DNA and promotes higher-order chromatin compaction. By regulating nucleosome spacing and local accessibility, Histone H1 influences genome-wide transcriptional programs, DNA replication timing, and DNA damage signaling within chromatin-associated pathways. Altered H1 stoichiometry or chromatin binding dynamics is implicated in epigenetic dysregulation observed across cancer and developmental disorders, making HIST1H1B a useful locus for studying chromatin state control and genome stability.
Histone H1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HIST1H1B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HIST1H1B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HIST1H1B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HIST1H1B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.