Histone cluster 1 H1E CRISPR/Cas9 KO Plasmid (h): sc-402775
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone cluster 1 H1E CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Histone cluster 1 H1E genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone cluster 1 H1E CRISPR/Cas9 KO Plasmid (h) | sc-402775 | 20 µg | $397.00 |
Overview
HIST1H1E encodes histone cluster 1 H1E, a replication-dependent linker histone H1 variant that binds nucleosomal DNA to stabilize higher-order chromatin structure and influence genome compaction. By modulating chromatin accessibility, H1E helps regulate transcriptional programs, DNA replication timing, and DNA damage responses through effects on chromatin remodeling and repair factor recruitment. Altered H1 family stoichiometry and linker-histone dynamics have been associated with epigenetic dysregulation observed in cancer and neurodevelopmental disorders, and HIST1H1E-related perturbations can impact cell fate decisions and genomic stability. As part of the Histone cluster 1 locus, HIST1H1E is also relevant for studies of S-phase–coupled histone expression and chromatin assembly.
Histone cluster 1 H1E CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HIST1H1E gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HIST1H1E together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HIST1H1E open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Histone cluster 1 H1E protein expression.
This CRISPR knockout system enables efficient generation of HIST1H1E-deficient cell models for investigation of Histone cluster 1 H1E signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HIST1H1E exon(s) critical for Histone cluster 1 H1E function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HIST1H1E genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Histone cluster 1 H1E CRISPR/Cas9 KO Plasmid (h) and Histone cluster 1 H1E CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HIST1H1E locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Histone cluster 1 H1E HDR Plasmid (h) and Histone cluster 1 H1E HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HIST1H1E homology arms to support homology-directed repair at defined HIST1H1E target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.