Histamine H4 Receptor CRISPR/Cas9 KO Plasmid (h): sc-402430
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histamine H4 Receptor CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Histamine H4 Receptor genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histamine H4 Receptor CRISPR/Cas9 KO Plasmid (h) | sc-402430 | 20 µg | $397.00 |
Overview
HRH4 encodes the histamine H4 receptor (H4R), a Gi/o-coupled GPCR predominantly expressed in hematopoietic and immune cell compartments where it senses histamine to regulate chemotaxis, cytokine release, and calcium-dependent signaling. Receptor engagement suppresses adenylyl cyclase activity, modulates cAMP levels, and interfaces with MAPK and PI3K-associated signaling networks that shape leukocyte migration and inflammatory tone. HRH4 activity contributes to immune cell trafficking and barrier-associated inflammation, linking histaminergic signaling to allergic and inflammatory processes. Dysregulated H4R signaling has been studied in contexts such as asthma, dermatitis, pruritus, and broader immune-mediated pathology, where receptor-dependent changes in chemokine responses and leukocyte recruitment are frequently evaluated.
Histamine H4 Receptor CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HRH4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HRH4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HRH4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Histamine H4 Receptor protein expression.
This CRISPR knockout system enables efficient generation of HRH4-deficient cell models for investigation of Histamine H4 Receptor signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HRH4 exon(s) critical for Histamine H4 Receptor function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HRH4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Histamine H4 Receptor CRISPR/Cas9 KO Plasmid (h) and Histamine H4 Receptor CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HRH4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Histamine H4 Receptor HDR Plasmid (h) and Histamine H4 Receptor HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HRH4 homology arms to support homology-directed repair at defined HRH4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.