Histamine H3 Receptor Antibody (D-5) recommended for detection of Histamine H3 Receptor of mouse, rat and human origin by WB, IP, IF and ELISA

Histamine H3 Receptor Antibody (D-5): sc-390140

Histamine H3 Receptor Antibody (D-5) is rated 4.5 out of 5 by 2.
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Datasheets

    • Anti-Histamine H3 Receptor Antibody (D-5) is a mouse monoclonal IgM (kappa light chain) Histamine H3 Receptor antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 301-405 near the C-terminus of Histamine H3 Receptor of human origin
    • recommended for detection of Histamine H3 Receptor of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and bovine
    • blocking peptide, sc-390140 P
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Histamine H3 Receptor (D-5): sc-390140.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

What application is the blocking peptide sc-390140 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Acceptable results in WB I used this antibody (1:100) in a WB experiment using whole cell lysates. A strong band at the predicted position was shown with several weak bands.
Date published: 2018-04-23
Rated 5 out of 5 by from Produced positive Western blot data of Histamine Produced positive Western blot data of Histamine H3 Receptor expression in A549, CHO-K1, C6, SK-N-MC and forskolin treated SK-N-MC whole cell lysates. -SCBT QC
Date published: 2014-08-19
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