Histamine H1 Receptor Double Nickase Plasmid (h): sc-401393-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histamine H1 Receptor Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Histamine H1 Receptor Double Nickase Plasmid (h) and Histamine H1 Receptor Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HRH1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histamine H1 Receptor Antibody (G-11): sc-374621
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histamine H1 Receptor Double Nickase Plasmid (h) | sc-401393-NIC | 20 µg | $410.00 | |||
Histamine H1 Receptor Double Nickase Plasmid (h2) | sc-401393-NIC-2 | 20 µg | $410.00 |
HRH1 encodes the human histamine H1 receptor (H1R), a class A G protein-coupled receptor primarily coupled to Gq/11 that transduces histamine signaling into phospholipase C activation, inositol phosphate generation, intracellular Ca2+ mobilization, and protein kinase C-dependent programs. H1R signaling intersects with MAPK/ERK and NF-κB pathways to regulate vascular tone and permeability, smooth muscle contraction, and inflammatory gene expression in immune and epithelial contexts. Altered HRH1 activity and expression has been associated with airway hyperresponsiveness, allergic inflammation, pruritus, and neuroimmune modulation, making it a useful node for studying histaminergic regulation of barrier and neuronal function. HRH1 is also leveraged as a model GPCR for investigating receptor desensitization, β-arrestin-mediated trafficking, and ligand-biased signaling.
Histamine H1 Receptor Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HRH1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HRH1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HRH1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HRH1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.