Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Histamine H1 Receptor Antibody (G-11): sc-374621. Western blot analysis of Histamine H1 Receptor expression in non-transfected (A) and human Histamine H1 Receptor transfected (B) HEK293T whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Histamine H1 Receptor CRISPR Activation Plasmid (h): sc-401393-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histamine H1 Receptor CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Histamine H1 Receptor CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Histamine H1 Receptor CRISPR Activation Plasmid (h) and Histamine H1 Receptor CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the HRH1 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histamine H1 Receptor Antibody (G-11): sc-374621
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histamine H1 Receptor CRISPR Activation Plasmid (h) | sc-401393-ACT | 20 µg | $397.00 | |||
Histamine H1 Receptor CRISPR Activation Plasmid (h2) | sc-401393-ACT-2 | 20 µg | $397.00 |