His-Tag Antibody (H-3): sc-8036
- His-Tag Antibody (H-3) is a mouse monoclonal IgG1 κ His-Tag antibody, cited in 537 publications, provided at 200 µg/ml
- raised against a His-tagged recombinant protein
- His-Tag Antibody (H-3) is recommended for detection of fusion proteins encoded by polyhistidine expression vectors by WB and IP
- Anti-His-Tag Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to biotin for WB, IHC(P) and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for His-Tag Antibody (H-3) for WB applications. These reagents are now offered in bundles with His-Tag Antibody (H-3) (see ordering information below).
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SEE ALSO...
His-Tag Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody raised against a His-tagged recombinant protein, designed for applications such as western blotting (WB) and immunoprecipitation (IP). The His-tag consists of a sequence of six histidine residues commonly fused to recombinant proteins to facilitate purification and detection. His-tagged proteins bind strongly to metal ions like nickel or cobalt, allowing efficient purification through immobilized metal affinity chromatography. While His-tags are not typically subject to post-translational modifications, fusion of His-tags to proteins can influence or be influenced by post-translational modifications of the target protein, such as phosphorylation, glycosylation, or ubiquitination. These modifications are crucial because they can affect protein function, localization, stability, and interactions within the cell. Understanding and detecting these changes is important for studying protein behavior in various biological processes and disease states. anti-His-Tag antibody (H-3) enables researchers to accurately detect and analyze His-tagged proteins, even when post-translational modifications are present, thus advancing research in protein expression, function, and therapeutic development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
His-Tag Antibody (H-3) References:
- Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs. | Els Conrath, K., et al. 2001. J Biol Chem. 276: 7346-50. PMID: 11053416
- The high mobility group protein HMG I(Y) is required for NF-kappa B-dependent virus induction of the human IFN-beta gene. | Thanos, D. and Maniatis, T. 1992. Cell. 71: 777-89. PMID: 1330326
- His-tag impact on structure. | Carson, M., et al. 2007. Acta Crystallogr D Biol Crystallogr. 63: 295-301. PMID: 17327666
- Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. | Smith, DB. and Johnson, KS. 1988. Gene. 67: 31-40. PMID: 3047011
- Large-scale chromatography of recombinant proteins. | Hochuli, E. 1988. J Chromatogr. 444: 293-302. PMID: 3060478
- Single-site phosphorylation within the His-tag sequence attached to a recombinant protein. | Singh, H., et al. 2019. Anal Biochem. 570: 62-64. PMID: 30735667
- Effects of His-tag on Catalytic Activity and Enantioselectivity of Recombinant Transaminases. | Meng, L., et al. 2020. Appl Biochem Biotechnol. 190: 880-895. PMID: 31515673
- Site-specific covalent labeling of His-tag fused proteins with N-acyl-N-alkyl sulfonamide reagent. | Thimaradka, V., et al. 2021. Bioorg Med Chem. 30: 115947. PMID: 33360195
- Highly Selective Lysine Acylation in Proteins Using a Lys-His Tag Sequence. | Kofoed, C., et al. 2022. Chemistry. 28: e202200147. PMID: 35099088
- Development of a Modular NTA:His Tag Viral Vaccine for Co-delivery of Antigen and Adjuvant. | Chung, YH., et al. 2023. Bioconjug Chem. 34: 269-278. PMID: 36608270
- Novel His-tag Variants for Insertion Inside Polypeptide Chain. | Tarabarova, AG., et al. 2024. ACS Omega. 9: 858-865. PMID: 38222536
- A multifunctional prokaryotic protein expression system: overproduction, affinity purification, and selective detection. | Kroll, DJ., et al. 1993. DNA Cell Biol. 12: 441-53. PMID: 7686011
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
His-Tag Antibody (H-3) | sc-8036 | 200 µg/ml | $316.00 | |||
His-Tag Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-526512 | 200 µg Ab; 10 µg BP | $354.00 | |||
His-Tag Antibody (H-3): m-IgG1 BP-HRP Bundle | sc-531885 | 200 µg Ab; 20 µg BP | $354.00 | |||
His-probe Antibody (H-3) AC | sc-8036 AC | 500 µg/ml, 25% agarose | $416.00 | |||
His-probe Antibody (H-3) HRP | sc-8036 HRP | 200 µg/ml | $316.00 | |||
His-probe Antibody (H-3) FITC | sc-8036 FITC | 200 µg/ml | $330.00 | |||
His-probe Antibody (H-3) PE | sc-8036 PE | 200 µg/ml | $343.00 | |||
His-probe Antibody (H-3) Alexa Fluor® 488 | sc-8036 AF488 | 200 µg/ml | $357.00 | |||
His-probe Antibody (H-3) Alexa Fluor® 546 | sc-8036 AF546 | 200 µg/ml | $357.00 | |||
His-probe Antibody (H-3) Alexa Fluor® 594 | sc-8036 AF594 | 200 µg/ml | $357.00 | |||
His-probe Antibody (H-3) Alexa Fluor® 647 | sc-8036 AF647 | 200 µg/ml | $357.00 | |||
His-probe Antibody (H-3) Alexa Fluor® 680 | sc-8036 AF680 | 200 µg/ml | $357.00 | |||
His-probe Antibody (H-3) Alexa Fluor® 790 | sc-8036 AF790 | 200 µg/ml | $357.00 | |||
His-probe Antibody (H-3) B | sc-8036 B | 200 µg/ml | $320.00 |