His-Tag Antibody (AD1.1.10): sc-53073
- His-Tag Antibody (AD1.1.10) is a mouse monoclonal IgG1 κ, cited in 66 publications, provided at 200 µg/ml
- raised against a 6x His-tagged polypeptide
- His-Tag Antibody (AD1.1.10) is recommended for detection of fusion proteins encoded by polyhistidine expression vectors by WB, IP and IF
- Anti-His-Tag Antibody (AD1.1.10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for His-Tag Antibody (AD1.1.10) for WB applications. These reagents are now offered in bundles with His-Tag Antibody (AD1.1.10) (see ordering information below).
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SEE ALSO...
His-Tag Antibody (AD1.1.10) is a mouse monoclonal IgG1 kappa light chain antibody raised against a 6x His-tagged polypeptide, designed for applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). The His-tag is a short sequence of six histidine residues added to either the N- or C-terminus of a protein of interest through genetic engineering techniques. This tag is crucial because it facilitates the purification and detection of recombinant proteins. The histidine residues have a strong affinity for metal ions like nickel and cobalt, allowing the tagged protein to bind efficiently to metal-chelating resins during affinity chromatography. This enables a single-step purification process, which is highly efficient and preserves the protein′s native structure and function. The small size of the His-tag minimizes interference with protein activity, making it an ideal choice for protein studies. Understanding and utilizing the His-tag is important for researchers working on protein expression, structure-function analyses, and the development of therapeutics, as it allows for easy isolation and characterization of proteins that may be difficult to study otherwise. Anti-His-Tag antibody (AD1.1.10) specifically binds to the His-tag sequence, enabling researchers to detect and analyze His-tagged proteins in various experimental applications, thereby advancing research in molecular biology, biochemistry, and related fields.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
His-Tag Antibody (AD1.1.10) | sc-53073 | 200 µg/ml | $322.00 | |||
His-Tag Antibody (AD1.1.10): m-IgG Fc BP-HRP Bundle | sc-526762 | 200 µg Ab; 10 µg BP | $361.00 | |||
His-Tag Antibody (AD1.1.10): m-IgG1 BP-HRP Bundle | sc-532135 | 200 µg Ab; 20 µg BP | $361.00 | |||
His-probe Antibody (AD1.1.10) AC | sc-53073 AC | 500 µg/ml, 25% agarose | $424.00 | |||
His-probe Antibody (AD1.1.10) HRP | sc-53073 HRP | 200 µg/ml | $322.00 | |||
His-probe Antibody (AD1.1.10) FITC | sc-53073 FITC | 200 µg/ml | $336.00 | |||
His-probe Antibody (AD1.1.10) PE | sc-53073 PE | 200 µg/ml | $349.00 | |||
His-probe Antibody (AD1.1.10) Alexa Fluor® 488 | sc-53073 AF488 | 200 µg/ml | $364.00 | |||
His-probe Antibody (AD1.1.10) Alexa Fluor® 546 | sc-53073 AF546 | 200 µg/ml | $364.00 | |||
His-probe Antibody (AD1.1.10) Alexa Fluor® 594 | sc-53073 AF594 | 200 µg/ml | $364.00 | |||
His-probe Antibody (AD1.1.10) Alexa Fluor® 647 | sc-53073 AF647 | 200 µg/ml | $364.00 | |||
His-probe Antibody (AD1.1.10) Alexa Fluor® 680 | sc-53073 AF680 | 200 µg/ml | $364.00 | |||
His-probe Antibody (AD1.1.10) Alexa Fluor® 790 | sc-53073 AF790 | 200 µg/ml | $364.00 |