HIRA Antibody (374C6a): sc-130636
- HIRA Antibody (374C6a) is a mouse monoclonal IgG2a HIRA antibody provided at 100 µg/ml
- raised against a recombinant protein corresponding an internal region of HIRA of human origin
- recommended for detection of HIRA of mouse, rat and human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HIRA Antibody (374C6a). This work is in progress.
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HIRA Antibody (374C6a) is a mouse monoclonal IgG2a antibody that detects HIRA protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-HIRA antibody (374C6a) is available as a non-conjugated format. HIRA protein plays a crucial role in cell cycle regulation, particularly during development, where HIRA is essential for controlling specific gene transcription programs. HIRA expression is notably high in regions containing neural crest cells, which are vital for the proper formation of various tissues and organs. HIRA structure includes seven copies of a WD repeat, which suggests a role in regulating histone gene expression, thereby influencing chromatin dynamics and gene accessibility. HIRA interacts with Cyclin dependent kinase 2 (Cdk-2) and Cyclin A at an RXL motif, leading to phosphorylation of the substrate at Thr 555, a modification critical for cell cycle progression. The gene encoding the 1017 amino acid HIRA protein is located on human chromosome 22q11, an area associated with DiGeorge Syndrome, a congenital disorder characterized by developmental defects linked to neural crest cell populations. HIRA′s ability to mediate these processes underscores HIRA′s importance in both normal development and potential pathologies, making HIRA (374C6a) antibody a valuable tool for researchers studying cell cycle dynamics and related diseases.
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HIRA Antibody (374C6a) References:
- HIRA, the human homologue of yeast Hir1p and Hir2p, is a novel cyclin-cdk2 substrate whose expression blocks S-phase progression. | Hall, C., et al. 2001. Mol Cell Biol. 21: 1854-65. PMID: 11238922
- Subnuclear localization and mitotic phosphorylation of HIRA, the human homologue of Saccharomyces cerevisiae transcriptional regulators Hir1p/Hir2p. | De Lucia, F., et al. 2001. Biochem J. 358: 447-55. PMID: 11513744
- A human homolog of the S. cerevisiae HIR1 and HIR2 transcriptional repressors cloned from the DiGeorge syndrome critical region. | Lamour, V., et al. 1995. Hum Mol Genet. 4: 791-9. PMID: 7633437
- Isolation of a putative transcriptional regulator from the region of 22q11 deleted in DiGeorge syndrome, Shprintzen syndrome and familial congenital heart disease. | Halford, S., et al. 1993. Hum Mol Genet. 2: 2099-107. PMID: 8111380
- Structural Organization of the WD repeat protein-encoding gene HIRA in the DiGeorge syndrome critical region of human chromosome 22. | Lorain, S., et al. 1996. Genome Res. 6: 43-50. PMID: 8681138
- HIRA, a DiGeorge syndrome candidate gene, is required for cardiac outflow tract septation. | Farrell, MJ., et al. 1999. Circ Res. 84: 127-35. PMID: 9933243
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIRA Antibody (374C6a) | sc-130636 | 100 µg/ml | $333.00 |