HIPK3 CRISPR/Cas9 KO Plasmid (h): sc-406669
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HIPK3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HIPK3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIPK3 CRISPR/Cas9 KO Plasmid (h) | sc-406669 | 20 µg | $397.00 |
Overview
Homeodomain-interacting protein kinase 3 (HIPK3) is a serine/threonine kinase that integrates stress and developmental cues by phosphorylating transcriptional regulators, thereby shaping gene expression programs linked to cell fate decisions. HIPK3 has been implicated in modulation of apoptotic signaling, DNA damage responses, and TGF-β/BMP-associated transcriptional outputs through interactions with homeobox proteins and other nuclear factors. Through these roles, HIPK3 can influence proliferation, differentiation, and cellular stress tolerance, processes frequently perturbed in cancer biology and tissue remodeling contexts. Dysregulated HIPK3 activity or expression has also been associated with altered inflammatory signaling and metabolic homeostasis, supporting its utility as a mechanistic node in pathway-centric studies.
HIPK3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HIPK3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HIPK3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HIPK3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HIPK3 protein expression.
This CRISPR knockout system enables efficient generation of HIPK3-deficient cell models for investigation of HIPK3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HIPK3 exon(s) critical for HIPK3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HIPK3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HIPK3 CRISPR/Cas9 KO Plasmid (h) and HIPK3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HIPK3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HIPK3 HDR Plasmid (h) and HIPK3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HIPK3 homology arms to support homology-directed repair at defined HIPK3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.