HIP12 Antibody (1C5): sc-58533
- HIP12 Antibody (1C5) is a mouse monoclonal IgG1 κ HIP12 antibody provided at 200 µg/ml
- raised against recombinant fragment HIP12 of human origin
- HIP12 Antibody (1C5) is recommended for detection of HIP12 of human origin, Hip1r of mouse origin and the corresponding rat homolog of mouse, rat and human origin by WB and IP
- Anti-HIP12 Antibody (1C5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HIP12 Antibody (1C5) for WB applications. These reagents are now offered in bundles with HIP12 Antibody (1C5) (see ordering information below).
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HIP12 Antibody (1C5) is a mouse monoclonal IgG1 kappa light chain antibody that detects HIP12 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). HIP12 (1C5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HIP12 protein plays a crucial role in the brain, where HIP12 is involved in maintaining membrane-cytoskeletal integrity, particularly in the context of Huntington′s disease. This neurodegenerative disorder is characterized by the expansion of a polyglutamine tract in the huntingtin protein, leading to disrupted interactions with huntingtin-interacting proteins like HIP1. While HIP12 does not directly bind to huntingtin, HIP12 shares a similar subcellular distribution with HIP1 and can interact with HIP1, suggesting a potential role in vesicle trafficking and cellular signaling pathways. The unique structural features of HIP12, including endocytic multidomain architecture, underscore HIP12′s importance in cellular processes that are vital for neuronal health. By utilizing anti-HIP12 antibody (1C5), researchers can explore the functional dynamics of HIP12 and its interactions, contributing to a deeper understanding of neurodegenerative mechanisms and potential therapeutic targets.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HIP12 Antibody (1C5) References:
- HIP12 is a non-proapoptotic member of a gene family including HIP1, an interacting protein with huntingtin. | Chopra, VS., et al. 2000. Mamm Genome. 11: 1006-15. PMID: 11063258
- The huntingtin interacting protein HIP1 is a clathrin and alpha-adaptin-binding protein involved in receptor-mediated endocytosis. | Waelter, S., et al. 2001. Hum Mol Genet. 10: 1807-17. PMID: 11532990
- HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. Identification of a novel interaction with clathrin light chain. | Legendre-Guillemin, V., et al. 2002. J Biol Chem. 277: 19897-904. PMID: 11889126
- Huntingtin interacting protein 1 (HIP1) regulates clathrin assembly through direct binding to the regulatory region of the clathrin light chain. | Legendre-Guillemin, V., et al. 2005. J Biol Chem. 280: 6101-8. PMID: 15533941
- HIP1, a human homologue of S. cerevisiae Sla2p, interacts with membrane-associated huntingtin in the brain. | Kalchman, MA., et al. 1997. Nat Genet. 16: 44-53. PMID: 9140394
- HIP-I: a huntingtin interacting protein isolated by the yeast two-hybrid system. | Wanker, EE., et al. 1997. Hum Mol Genet. 6: 487-95. PMID: 9147654
- Localization of the human HIP1 gene close to the elastin (ELN) locus on 7q11.23. | Wedemeyer, N., et al. 1997. Genomics. 46: 313-5. PMID: 9417924
- IRS-PCR-based genetic mapping of the huntingtin interacting protein gene (HIP1) on mouse chromosome 5. | Himmelbauer, H., et al. 1998. Mamm Genome. 9: 26-31. PMID: 9434941
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIP12 Antibody (1C5) | sc-58533 | 200 µg/ml | $322.00 | |||
HIP12 Antibody (1C5): m-IgG Fc BP-HRP Bundle | sc-526908 | 200 µg Ab; 10 µg BP | $361.00 | |||
HIP12 Antibody (1C5): m-IgG1 BP-HRP Bundle | sc-532281 | 200 µg Ab; 20 µg BP | $361.00 | |||
HIP12 Antibody (1C5) AC | sc-58533 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HIP12 Antibody (1C5) HRP | sc-58533 HRP | 200 µg/ml | $322.00 | |||
HIP12 Antibody (1C5) FITC | sc-58533 FITC | 200 µg/ml | $336.00 | |||
HIP12 Antibody (1C5) PE | sc-58533 PE | 200 µg/ml | $349.00 | |||
HIP12 Antibody (1C5) Alexa Fluor® 488 | sc-58533 AF488 | 200 µg/ml | $364.00 | |||
HIP12 Antibody (1C5) Alexa Fluor® 546 | sc-58533 AF546 | 200 µg/ml | $364.00 | |||
HIP12 Antibody (1C5) Alexa Fluor® 594 | sc-58533 AF594 | 200 µg/ml | $364.00 | |||
HIP12 Antibody (1C5) Alexa Fluor® 647 | sc-58533 AF647 | 200 µg/ml | $364.00 | |||
HIP12 Antibody (1C5) Alexa Fluor® 680 | sc-58533 AF680 | 200 µg/ml | $364.00 | |||
HIP12 Antibody (1C5) Alexa Fluor® 790 | sc-58533 AF790 | 200 µg/ml | $364.00 |