HiNF-P Antibody (C-5): sc-373855

HiNF-P Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects HiNF-P of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HiNF-P (C-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HiNF-P plays a critical role as a transcription factor essential for the E2F-independent activation of the Histone H4 multigene family, which is vital for proper cell cycle regulation and gene expression during cell division. Located primarily in the nucleus, HiNF-P interacts with conserved H4 cell cycle regulatory sequences, facilitating the transcription of Histone H4 genes. This interaction ensures that histone proteins are synthesized in coordination with DNA replication, thereby maintaining chromatin structure and function. Notably, reduction of HiNF-P levels through antisense inhibition leads to decreased expression of endogenous Histone H4, highlighting HiNF-P′s importance in cell cycle progression. Research has shown that HiNF-P acts as a key link in a signaling pathway initiated by growth factor-dependent induction of cyclin E/cyclin-dependent kinase 2 activity, ultimately leading to the activation of Histone H4 genes at the G1/S phase transition.

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HiNF-P Antibody (C-5) References:

1. Maintenance of open chromatin and selective genomic occupancy at the cell cycle-regulated histone H4 promoter during differentiation of HL-60 promyelocytic leukemia cells.  |  Hovhannisyan, H., et al. 2003. Mol Cell Biol. 23: 1460-9. PMID: 12556504
2. Identification of HiNF-P, a key activator of cell cycle-controlled histone H4 genes at the onset of S phase.  |  Mitra, P., et al. 2003. Mol Cell Biol. 23: 8110-23. PMID: 14585971
3. Regulation of Rb gene expression by an MBD2-interacting zinc finger protein MIZF during myogenic differentiation.  |  Sekimata, M. and Homma, Y. 2004. Biochem Biophys Res Commun. 325: 653-9. PMID: 15541338
4. HiNF-P directly links the cyclin E/CDK2/p220NPAT pathway to histone H4 gene regulation at the G1/S phase cell cycle transition.  |  Miele, A., et al. 2005. Mol Cell Biol. 25: 6140-53. PMID: 15988025
5. Coordinate control and selective expression of the full complement of replication-dependent histone H4 genes in normal and cancer cells.  |  Holmes, WF., et al. 2005. J Biol Chem. 280: 37400-7. PMID: 16131487
6. Transcriptional element H4-site II of cell cycle regulated human H4 histone genes is a multipartite protein/DNA interaction site for factors HiNF-D, HiNF-M, and HiNF-P: involvement of phosphorylation.  |  van Wijnen, AJ., et al. 1991. J Cell Biochem. 46: 174-89. PMID: 1655821
7. An architectural perspective of cell-cycle control at the G1/S phase cell-cycle transition.  |  Stein, GS., et al. 2006. J Cell Physiol. 209: 706-10. PMID: 17001681
8. Concerted control of multiple histone promoter factors during cell density inhibition of proliferation in osteosarcoma cells: reciprocal regulation of cell cycle-controlled and bone-related genes.  |  van den Ent, FM., et al. 1993. Cancer Res. 53: 2399-409. PMID: 8485727
9. The integrated activities of IRF-2 (HiNF-M), CDP/cut (HiNF-D) and H4TF-2 (HiNF-P) regulate transcription of a cell cycle controlled human histone H4 gene: mechanistic differences between distinct H4 genes.  |  Aziz, F., et al. 1998. Mol Biol Rep. 25: 1-12. PMID: 9540062
10. HiNF-D (CDP-cut/CDC2/cyclin A/pRB-complex) influences the timing of IRF-2-dependent cell cycle activation of human histone H4 gene transcription at the G1/S phase transition.  |  Aziz, F., et al. 1998. J Cell Physiol. 177: 453-64. PMID: 9808153

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HiNF-P Antibody (C-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-HiNF-P Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HiNF-P Antibody (C-5) Specifications

Alternate Product Name: HiNF-P

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 64-270

Epitope Target: HiNF-P

Epitope Species: human

Product Reactivity/Detection: HiNF-P

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for HiNF-P Antibody (C-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent