HIBADH Antibody (D-11): sc-398288

HIBADH Antibody (D-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects HIBADH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-HIBADH antibody (D-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HIBADH (3-hydroxyisobutyrate dehydrogenase) is a crucial mitochondrial enzyme composed of 336 amino acids that plays a significant role in valine catabolism by catalyzing the NAD+-dependent reversible oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde. This enzymatic activity maintains metabolic homeostasis, particularly in energy production and amino acid metabolism. HIBADH functions as a homodimer, exhibiting optimal activity at a pH range of 8.8 to 9.0, which supports mitochondrial function where pH levels can vary. While defects in HIBADH were once thought to contribute to 3-hydroxyisobutyric aciduria, a rare metabolic disorder, studies have shown that HIBADH activity remains consistent in affected individuals compared to healthy controls, highlighting the complexity of metabolic disorders and the need for further research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HIBADH Antibody (D-11) References:

1. Crystallization and preliminary X-ray crystallographic studies of NADP-dependent 3-hydroxyisobutyrate dehydrogenase from Thermus thermophilus HB8.  |  Lokanath, NK., et al. 2003. Acta Crystallogr D Biol Crystallogr. 59: 2294-6. PMID: 14646099
2. Conserved expression domains for genes upstream and within the HoxA and HoxD clusters suggests a long-range enhancer existed before cluster duplication.  |  Lehoczky, JA., et al. 2004. Evol Dev. 6: 423-30. PMID: 15509224
3. Crystal structure of novel NADP-dependent 3-hydroxyisobutyrate dehydrogenase from Thermus thermophilus HB8.  |  Lokanath, NK., et al. 2005. J Mol Biol. 352: 905-17. PMID: 16126223
4. Clinical, biochemical, and molecular findings in three patients with 3-hydroxyisobutyric aciduria.  |  Loupatty, FJ., et al. 2006. Mol Genet Metab. 87: 243-8. PMID: 16466957
5. Expression of 3-hydroxyisobutyrate dehydrogenase in cultured neural cells.  |  Murín, R., et al. 2008. J Neurochem. 105: 1176-86. PMID: 18284611
6. [Cloning of silkworm 3-hydroxyisobutyrate dehydrogenase gene and its expression patterns analysis in simulated weightless environment].  |  Tian, Z., et al. 2008. Sheng Wu Gong Cheng Xue Bao. 24: 2041-8. PMID: 19306573
7. The catalytic property of 3-hydroxyisobutyrate dehydrogenase from Bacillus cereus on 3-hydroxypropionate.  |  Yao, T., et al. 2010. Appl Biochem Biotechnol. 160: 694-703. PMID: 19517068
8. Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker.  |  Tasi, YC., et al. 2013. J Assist Reprod Genet. 30: 505-12. PMID: 23423614
9. Cloning and sequence analysis of a cDNA for 3-hydroxyisobutyrate dehydrogenase. Evidence for its evolutionary relationship to other pyridine nucleotide-dependent dehydrogenases.  |  Rougraff, PM., et al. 1989. J Biol Chem. 264: 5899-903. PMID: 2647728
10. 3-Hydroxyisobutyrate dehydrogenase (HIBADH) deficiency-A novel disorder of valine metabolism.  |  Meyer, M., et al. 2021. J Inherit Metab Dis. 44: 1323-1329. PMID: 34176136
11. Lnc-HIBADH-4 Regulates Autophagy-Lysosome Pathway in Amyotrophic Lateral Sclerosis by Targeting Cathepsin D.  |  Huang, J., et al. 2024. Mol Neurobiol. 61: 4768-4782. PMID: 38135852
12. Shared genetic correlations between kidney diseases and sepsis.  |  Zhang, T., et al. 2024. Front Endocrinol (Lausanne). 15: 1396041. PMID: 39086896

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HIBADH Antibody (D-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HIBADH Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HIBADH Antibody (D-11) Specifications

Alternate Product Name: HIBADH

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-157

Epitope Target: HIBADH

Epitope Species: human

Product Reactivity/Detection: HIBADH

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HIBADH Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin