Hhip Antibody (5D11): sc-293265

Hhip Antibody (5D11) is a mouse monoclonal IgG2b kappa light chain antibody that detects Hhip protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Hhip antibody (5D11) is available as the non-conjugated form. Hhip plays a crucial role in regulating Hedgehog signaling, which is essential for proper growth and tissue patterning during embryonic development. Located on the cell surface, Hhip interacts with Hedgehog proteins, including Sonic hedgehog, to modulate their activity. This interaction helps prevent excessive signaling that can lead to developmental abnormalities and diseases such as cancer. By binding to Hedgehog proteins, Hhip acts as a negative regulator, ensuring that signaling pathways remain finely tuned. Hhip′s influence on Hedgehog signaling highlights its importance in developmental biology and potential as a therapeutic target in conditions where this pathway is dysregulated.

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Hhip Antibody (5D11) References:

1. Vertebrate Hedgehog signalling modulated by induction of a Hedgehog-binding protein.  |  Chuang, PT. and McMahon, AP. 1999. Nature. 397: 617-21. PMID: 10050855
2. Hhip haploinsufficiency sensitizes mice to age-related emphysema.  |  Lao, T., et al. 2016. Proc Natl Acad Sci U S A. 113: E4681-7. PMID: 27444019
3. Hedgehog Interacting Protein (Hhip) Regulates Insulin Secretion in Mice Fed High Fat Diets.  |  Nchienzia, H., et al. 2019. Sci Rep. 9: 11183. PMID: 31371780
4. Hedgehog interacting protein (HHIP) represses airway remodeling and metabolic reprogramming in COPD-derived airway smooth muscle cells.  |  Li, Y., et al. 2021. Sci Rep. 11: 9074. PMID: 33907231
5. The HHIP-AS1 lncRNA promotes tumorigenicity through stabilization of dynein complex 1 in human SHH-driven tumors.  |  Bartl, J., et al. 2022. Nat Commun. 13: 4061. PMID: 35831316
6. lncRNA HHIP-AS1/HHIP modulates osteogenic differentiation of BM-MSCs by regulating Hedgehog signaling pathway.  |  Yin, XH., et al. 2022. Aging (Albany NY). 14: 8839-8855. PMID: 36375472
7. LncRNA HHIP-AS1 suppresses lung squamous cell carcinoma by stabilizing HHIP mRNA.  |  Hu, Z., et al. 2023. Life Sci. 321: 121578. PMID: 36958438
8. Conservation of the hedgehog/patched signaling pathway from flies to mice: induction of a mouse patched gene by Hedgehog.  |  Goodrich, LV., et al. 1996. Genes Dev. 10: 301-12. PMID: 8595881
9. Regulation of patched by sonic hedgehog in the developing neural tube.  |  Marigo, V. and Tabin, CJ. 1996. Proc Natl Acad Sci U S A. 93: 9346-51. PMID: 8790332
10. The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog.  |  Stone, DM., et al. 1996. Nature. 384: 129-34. PMID: 8906787
11. The role of sonic hedgehog in vertebrate development.  |  Weed, M., et al. 1997. Matrix Biol. 16: 53-8. PMID: 9205942
12. Ptch2, a second mouse Patched gene is co-expressed with Sonic hedgehog.  |  Motoyama, J., et al. 1998. Nat Genet. 18: 104-6. PMID: 9462734

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Hhip Antibody (5D11) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Hhip Antibody (5D11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Hhip Antibody (5D11) Specifications

Alternate Product Name: Hhip

Product Clone Name: 5D11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 21-120

Epitope Target: Hhip

Epitope Species: human

Product Reactivity/Detection: Hhip

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Hhip Antibody (5D11): N/A