Date published: 2026-5-28

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HGFα Antibody (B-3): sc-166724

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Datasheets
  • HGFα Antibody (B-3) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 32-176 of HGFα of human origin
  • recommended for detection of HGFα of human origin by WB, IP, IF and ELISA
  • See HGFα (H-10): sc-374422 for HGFα antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for HGFα Antibody (B-3) for WB applications. These reagents are now offered in bundles with HGFα Antibody (B-3) (see ordering information below).
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HGFα Antibody (B-3) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect the alpha subunit of hepatocyte growth factor (HGFα), also known as scatter factor, hematopoietin A, or mammary growth factor. HGFα is a critical component of the HGF protein, which plays a pivotal role in various cellular processes such as growth, motility, and morphogenesis by binding to its primary receptor, c-Met. This interaction is essential for normal physiological functions including liver regeneration, wound healing, and embryonic development. Additionally, HGFα interacts with several other proteins, including integrins and extracellular matrix components, which facilitate its binding to c-Met and enhance signal transduction pathways that regulate cell proliferation and survival. HGFα monoclonal antibody (B-3) is raised against amino acids 32-176 of human HGFα, ensuring high specificity and affinity for the target protein. Anti-HGFα antibody (B-3) is validated for use in multiple applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), making it a versatile tool for researchers studying HGFα-related pathways. Importantly, anti-HGFα antibody (B-3) does not cross-react with other tested cytokines in indirect enzyme-linked immunosorbent assay (ELISA), providing reliable and accurate results in your experiments. By enabling precise detection and quantification of HGFα, anti-HGFα antibody (B-3) supports the investigation of HGF-mediated signaling mechanisms and their roles in both normal physiology and pathological conditions such as cancer, where HGFα can promote tumor growth and metastasis.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

HGFα Antibody (B-3)

sc-166724
200 µg/ml
$322.00

HGFα Antibody (B-3): m-IgG Fc BP-HRP Bundle

sc-540147
200 µg Ab; 10 µg BP
$361.00

HGFα Antibody (B-3): m-IgG2a BP-HRP Bundle

sc-546858
200 µg Ab; 10 µg BP
$361.00

What is the recommended starting dilution for Western blot for sc-166724: HGFα (B-3) monoclonal antibody?

Asked by: Dr Ninau Qelp
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from 抗体非常好用之前用的其他抗体,显不出来,现在用的你们公司的产品,显出的条带非常好,很好用,希望越做越好,生意兴隆。
Date published: 2017-06-23
Rated 5 out of 5 by from Produced publishable WB dataThis HGF alpha monoclonal antibody was featured in a recent publication for the use of WB in MCF10DCIS cells
Date published: 2017-01-23
Rated 5 out of 5 by from Nice Western blot data of full length humanNice Western blot data of full length human recombinant HGF . -SCBT QC
Date published: 2013-07-01
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HGFα Antibody (B-3) is rated 5.0 out of 5 by 3.
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