HGFA Antibody (B-6): sc-515126

HGFA Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects HGFA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HGFA (B-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hepatocyte growth factor activator (HGFA) plays a crucial role in the activation of hepatocyte growth factor (HGF), a key player in tissue regeneration and repair. HGFA is a serine protease that specifically cleaves the inactive single-chain precursor of HGF into its biologically active heterodimeric form, which consists of an α chain and a β chain linked by disulfide bonds. This activation is vital as HGF exerts its effects through the c-Met receptor, influencing cellular processes such as proliferation, migration, and morphogenesis, particularly in liver and epithelial tissues. The gene encoding HGFA is located on human chromosome 4p16, and HGFA is synthesized as a 655 amino acid precursor that is secreted in an inactive form, later processed to yield a 35 amino acid short chain and a 248 amino acid long chain. The specificity of HGFA to the liver underscores its importance in hepatic function and regeneration, making anti-HGFA antibody (B-6) an essential tool for researchers studying liver biology and related pathologies.

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HGFA Antibody (B-6) References:

1. HGF/SF: a potent cytokine for mammary growth, morphogenesis and development.  |  Niranjan, B., et al. 1995. Development. 121: 2897-908. PMID: 7555716
2. Molecular cloning and sequence analysis of the cDNA for a human serine protease reponsible for activation of hepatocyte growth factor. Structural similarity of the protease precursor to blood coagulation factor XII.  |  Miyazawa, K., et al. 1993. J Biol Chem. 268: 10024-8. PMID: 7683665
3. Biological activation of pro-HGF (hepatocyte growth factor) by urokinase is controlled by a stoichiometric reaction.  |  Naldini, L., et al. 1995. J Biol Chem. 270: 603-11. PMID: 7822285
4. The Met/HGF receptor is over-expressed in human osteosarcomas and is activated by either a paracrine or an autocrine circuit.  |  Ferracini, R., et al. 1995. Oncogene. 10: 739-49. PMID: 7862451
5. Proteolytic activation of hepatocyte growth factor in response to tissue injury.  |  Miyazawa, K., et al. 1994. J Biol Chem. 269: 8966-70. PMID: 8132634
6. Activation of the zymogen of hepatocyte growth factor activator by thrombin.  |  Shimomura, T., et al. 1993. J Biol Chem. 268: 22927-32. PMID: 8226803
7. Coexpression of hepatocyte growth factor and receptor (Met) in human breast carcinoma.  |  Tuck, AB., et al. 1996. Am J Pathol. 148: 225-32. PMID: 8546209
8. Structural organization and chromosomal localization of the human hepatocyte growth factor activator gene--phylogenetic and functional relationship with blood coagulation factor XII, urokinase, and tissue-type plasminogen activator.  |  Miyazawa, K., et al. 1998. Eur J Biochem. 258: 355-61. PMID: 9874200

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HGFA Antibody (B-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-HGFA Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HGFA Antibody (B-6) Specifications

Alternate Product Name: HGFA

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 464-580

Epitope Target: HGFA

Epitope Species: human

Product Reactivity/Detection: HGFA precursor and long chain

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for HGFA Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin