Hex Double Nickase Plasmid (h): sc-403827-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hex Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Hex Double Nickase Plasmid (h) and Hex Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HHEX. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Hex Antibody (HHE5I261): sc-81284
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hex Double Nickase Plasmid (h) | sc-403827-NIC | 20 µg | $410.00 | |||
Hex Double Nickase Plasmid (h2) | sc-403827-NIC-2 | 20 µg | $410.00 |
Human HHEX encodes Hex, a homeobox transcription factor that coordinates lineage specification and organogenesis, with prominent roles in endoderm patterning and early hematopoietic development. Hex integrates context-dependent transcriptional programs that influence cell fate decisions, proliferation, and differentiation through regulatory networks intersecting with developmental signaling pathways such as Wnt, BMP, and TGF-β. In mature tissues, HHEX contributes to vascular and immune cell biology, and dysregulated expression has been linked to altered differentiation states and oncogenic transcriptional circuitry in several malignancies. Because HHEX functions as a node in developmental and hematopoietic gene regulatory networks, it is frequently interrogated to map enhancer usage, transcriptional targets, and context-specific chromatin states.
Hex Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HHEX locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HHEX. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HHEX function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HHEX-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.