Hex CRISPR/Cas9 KO Plasmid (m): sc-420852
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hex CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Hex genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hex CRISPR/Cas9 KO Plasmid (m) | sc-420852 | 20 µg | $397.00 |
Overview
Hhex (Hex) is a homeobox transcription factor that regulates early embryonic patterning and organogenesis, with prominent functions in anterior endoderm specification and the development of liver, pancreas, and thyroid lineages in mouse. By binding DNA through its homeodomain, Hex integrates developmental signaling inputs to control gene expression programs that influence cell fate decisions, morphogenesis, and tissue differentiation. Hhex activity intersects with pathways that govern endodermal and vascular development and has been linked to the regulation of hematopoietic and endothelial gene networks. Dysregulated Hhex expression or function is associated with developmental abnormalities and has been implicated in altered differentiation states relevant to cancer biology and hematologic disease models.
Hex CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hhex gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hhex together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hhex open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Hex protein expression.
This CRISPR knockout system enables efficient generation of Hhex-deficient cell models for investigation of Hex signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hhex exon(s) critical for Hex function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hhex genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Hex CRISPR/Cas9 KO Plasmid (m) and Hex CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hhex locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Hex HDR Plasmid (m) and Hex HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hhex homology arms to support homology-directed repair at defined Hhex target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.