HES7 CRISPR/Cas9 KO Plasmid (h): sc-407096
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HES7 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HES7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HES7 CRISPR/Cas9 KO Plasmid (h) | sc-407096 | 20 µg | $397.00 |
Overview
HES7 (hairy and enhancer of split 7) encodes a bHLH transcriptional repressor that functions as a core component of the segmentation clock during human embryonic development. It operates downstream of Notch signaling and integrates oscillatory gene expression programs that coordinate somitogenesis, with tight regulation of transcriptional timing and negative feedback dynamics. Altered HES7 activity disrupts somite patterning and has been linked to congenital vertebral segmentation abnormalities, making it relevant for studying developmental gene regulatory networks. In cellular models, HES7 is commonly examined for its roles in transcriptional repression, Notch pathway crosstalk, and rhythmic expression control mechanisms.
HES7 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HES7 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HES7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HES7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HES7 protein expression.
This CRISPR knockout system enables efficient generation of HES7-deficient cell models for investigation of HES7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HES7 exon(s) critical for HES7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HES7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HES7 CRISPR/Cas9 KO Plasmid (h) and HES7 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HES7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HES7 HDR Plasmid (h) and HES7 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HES7 homology arms to support homology-directed repair at defined HES7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.