HES3 CRISPR/Cas9 KO Plasmid (h): sc-406281
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HES3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HES3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HES3 CRISPR/Cas9 KO Plasmid (h) | sc-406281 | 20 µg | $397.00 |
Overview
HES3 (hairy and enhancer of split 3) is a basic helix–loop–helix transcriptional repressor that functions downstream of Notch signaling to modulate gene expression programs controlling cell fate decisions. It contributes to maintenance of undifferentiated states and regulation of lineage commitment by antagonizing proneural and differentiation-associated transcription factors, integrating cues from developmental pathways. In human cells, altered HES family activity has been linked to dysregulated differentiation, aberrant proliferation, and stem-like phenotypes in disease-relevant contexts, making HES3 a useful node for studying transcriptional control of developmental programs. Its roles in neurodevelopmental and progenitor-associated gene networks support investigation of signaling-to-transcription coupling and epigenetic regulation.
HES3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HES3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HES3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HES3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HES3 protein expression.
This CRISPR knockout system enables efficient generation of HES3-deficient cell models for investigation of HES3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HES3 exon(s) critical for HES3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HES3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HES3 CRISPR/Cas9 KO Plasmid (h) and HES3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HES3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HES3 HDR Plasmid (h) and HES3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HES3 homology arms to support homology-directed repair at defined HES3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.