Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HERG Double Nickase Plasmid (h): sc-401143-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HERG Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HERG Double Nickase Plasmid (h) and HERG Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KCNH2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HERG Antibody (F-12): sc-377388
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HERG Double Nickase Plasmid (h) | sc-401143-NIC | 20 µg | $410.00 | |||
HERG Double Nickase Plasmid (h2) | sc-401143-NIC-2 | 20 µg | $410.00 |
KCNH2 encodes the human HERG (Kv11.1) voltage-gated potassium channel that conducts the rapid delayed rectifier current (IKr), a major determinant of cardiac action potential repolarization and QT interval duration. HERG channel activity integrates into excitability and ion homeostasis pathways by shaping membrane potential dynamics and refractory period timing. Genetic or functional perturbation of KCNH2 is associated with inherited and acquired long QT phenotypes and ventricular arrhythmia susceptibility, and the channel is a frequent off-target liability in electrophysiology and safety pharmacology studies. Beyond cardiomyocytes, KCNH2 expression in excitable and proliferative cell contexts supports research into channel trafficking, gating regulation, and membrane-signaling crosstalk.
HERG Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KCNH2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KCNH2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KCNH2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KCNH2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.