Hepsin Double Nickase Plasmid (h): sc-405081-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hepsin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Hepsin Double Nickase Plasmid (h) and Hepsin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HPN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Hepsin Antibody (2D5): sc-517056
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hepsin Double Nickase Plasmid (h) | sc-405081-NIC | 20 µg | $410.00 | |||
Hepsin Double Nickase Plasmid (h2) | sc-405081-NIC-2 | 20 µg | $410.00 |
HPN encodes hepsin, a type II transmembrane serine protease enriched at the cell surface where it participates in pericellular proteolysis, extracellular matrix remodeling, and regulation of cell–cell and cell–matrix interactions. Hepsin can influence protease-activated signaling and growth factor availability by processing extracellular substrates, linking it to pathways that shape epithelial differentiation, tissue architecture, and invasive behavior. Dysregulated HPN expression and altered hepsin activity have been reported in multiple epithelial malignancies, making it a useful molecular handle for studying tumor-associated protease networks and microenvironmental crosstalk. In biomedical research, HPN is frequently examined for its roles in membrane-anchored protease cascades, cell migration, and proteostasis at the plasma membrane.
Hepsin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HPN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HPN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HPN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HPN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.