hepcidin Antibody (56-Z) recommended for detection of hepcidin of human origin by IF, IHC(P) and ELISA

hepcidin Antibody (56-Z): sc-100277

hepcidin Antibody (56-Z) is rated 4.5 out of 5 by 2.
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Datasheets

    • Anti-hepcidin Antibody (56-Z) is a mouse monoclonal IgG1 (kappa light chain) hepcidin antibody provided at 100 µg/ml
    • raised against full-length recombinant protein mapping within amino acids 25-85 of hepcidin of human origin
    • recommended for detection of hepcidin of human origin by IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

Dear technique support, I have bought the antibody of hepcidin(56-Z): sc-100277, I want to do immunohistochemistry, can you tell me the protocol that you used it to do immunohistochemistry? Thank you very much!

Asked by: thrust han
Thank you for your question. You can find our recommended protocol for IHC applications by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunoperoxidase-staining
Answered by: Tech Support Europe
Date published: 2020-08-28

Is there trial size of hepcidin(56-Z): sc-100277? I want to measure the effect of it firstly.

Asked by: thrust han
Thank you for your question. We do not offer a trial size of this antibody, unfortunately. However, it is recommended and warrantied for detection of hepcidin of human origin by IF, IHC(P) and ELISA, so there is no risk involved if you decide to purchase it.
Answered by: Tech Support Europe
Date published: 2020-08-04

Hi Is this antibody only specific for human or just not tested for other species (e.g. mouse & rat)? In case not tested, is it possible to obtain a trial vial to test the antibody on both rat and mice liver specimens?

Asked by: Bassem
Thank you for your inquiry and your interest in this product. We have not tested the hepcidin Antibody (56-Z): sc-100277 on mouse or rat. Thus we do not recommend nor warrant it for the detection of hepcidin from mouse or rat origin. We do not offer this antibody in a sample size. For further questions please contact our European Support Office in Germany directly. You can reach us by phone at: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com We are happy to assist you.
Answered by: Technical Support Europe
Date published: 2017-12-15

Does it work on mouse hepcidin?

Asked by: Deliang
Thank you for your question. This antibody, sc-100277 Hepcidin (56-Z) has not been tested for mouse and is therefore not recommended nor warrantied to use on mouse samples. It may work, but we can not recommend that it will do so. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-06

Can sc-100277: hepcidin (56-Z) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, sc-100277: hepcidin (56-Z) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Great I used this antibody to detect the expression of hepcidin in section of liver tissue.
Date published: 2016-12-05
Rated 4 out of 5 by from Produced wonderful immunoperoxidase cytoplasmic Produced wonderful immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2014-12-08
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