Hepatocyte Specific Antigen Antibody (OCH1E5): sc-58693

Hepatocyte Specific Antigen Antibody (OCH1E5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Hepatocyte Specific Antigen protein of human origin by immunofluorescence (IF) and immunohistochemistry with paraffin-embedded sections (IHCP). Hepatocyte Specific Antigen (OCH1E5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hepatocyte Specific Antigen, also known as Hepatocyte Paraffin 1 or Hep Par 1, is primarily localized to the mitochondria of hepatocytes, which is crucial for its role in cellular metabolism and energy production. This localization is significant because Hepatocyte Specific Antigen helps diagnose hepatocellular carcinomas (HCC) in humans and dogs, while distinguishing these tumors from metastatic carcinomas and cholangiocarcinomas. Strong expression of Hepatocyte Specific Antigen is associated with smaller tumor sizes and improved patient survival rates. While HCCs predominantly occur in the liver, these tumors can also manifest in other organs, and Hepatocyte Specific Antigen may serve as a valuable marker for intestinal metaplasia. A small percentage of HCCs may lack Hepatocyte Specific Antigen, which could indicate an alternative mechanism of hepatocarcinogenesis in the early stages of disease. Hepatocyte Specific Antigen is found to a lesser extent in gastric carcinomas and a few other non-hepatic tumors, further highlighting Hepatocyte Specific Antigen′s relevance in cancer diagnostics.

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Hepatocyte Specific Antigen Antibody (OCH1E5) References:

1. Hep par 1 antibody stain for the differential diagnosis of hepatocellular carcinoma: 676 tumors tested using tissue microarrays and conventional tissue sections.  |  Fan, Z., et al. 2003. Mod Pathol. 16: 137-44. PMID: 12591966
2. Immunohistochemical characterization of canine intestinal epithelial and mesenchymal tumours with a monoclonal antibody to hepatocyte paraffin 1 (Hep Par 1).  |  Ramos-Vara, JA. and Miller, MA. 2002. Histochem J. 34: 397-401. PMID: 12814187
3. Hepatocyte paraffin 1 antibody does not distinguish primary ovarian tumors with hepatoid differentiation from metastatic hepatocellular carcinoma.  |  Pitman, MB., et al. 2004. Int J Gynecol Pathol. 23: 58-64. PMID: 14668552
4. Hepatocyte paraffin 1 expression in human normal and neoplastic tissues: tissue microarray analysis on 3,940 tissue samples.  |  Lugli, A., et al. 2004. Am J Clin Pathol. 122: 721-7. PMID: 15491968
5. Immunohistological evaluation of single small hepatocellular carcinoma with negative staining of monoclonal antibody Hepatocyte Paraffin 1.  |  Sugiki, T., et al. 2004. J Surg Oncol. 88: 104-7. PMID: 15499598
6. Hepatoid carcinoma of the ovary: characteristics of its immunoreactivity. A case report.  |  Tsung, JS. and Yang, PS. 2004. Eur J Gynaecol Oncol. 25: 745-8. PMID: 15597858
7. Expression of Ep-CAM in intestinal metaplasia, gastric epithelial dysplasia and gastric adenocarcinoma.  |  Joo, M., et al. 2005. J Gastroenterol Hepatol. 20: 1039-45. PMID: 15955212
8. Elevated hepatocyte paraffin 1 and neprilysin expression in hepatocellular carcinoma are correlated with longer survival.  |  Mondada, D., et al. 2006. Virchows Arch. 448: 35-45. PMID: 16220294
9. The binding of thyroid transcription factor-1 and hepatocyte paraffin 1 to mitochondrial proteins in hepatocytes: a molecular and immunoelectron microscopic study.  |  Pang, Y., et al. 2006. Am J Clin Pathol. 125: 722-6. PMID: 16707373
10. Utilization of antihepatocyte clone OCH1E5 (Hep Par 1) in histological evaluation of liver tumors.  |  Amarapurkar, AD., et al. 2006. Indian J Pathol Microbiol. 49: 341-4. PMID: 17001880
11. Hepatocyte paraffin 1 and arginase-1 are effective panel of markers in HBV-related HCC diagnosis in fine-needle aspiration specimens.  |  Moudi, B., et al. 2020. BMC Res Notes. 13: 388. PMID: 32854754
12. Hepatocyte paraffin-1, CD10, and CD34 immunostaining as a diagnostic aid in cytologic diagnosis of hepatic cancer.  |  Agarwal, A., et al. 2022. J Cancer Res Ther. 18: S434-S438. PMID: 36510999

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Hepatocyte Specific Antigen Antibody (OCH1E5) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-Hepatocyte Specific Antigen Antibody (OCH1E5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Hepatocyte Specific Antigen Antibody (OCH1E5) Specifications

Alternate Product Name: Hepatocyte Specific Antigen

Product Clone Name: OCH1E5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Hepatocyte Specific Antigen

Epitope Species: human

Product Reactivity/Detection: an uncharacterized antigen present in both adults and fetal normal hepatocytes to produce a distinct granular cytoplasmic staining. This antibody stains the majority of hepatocellular carcinomas

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for Hepatocyte Specific Antigen Antibody (OCH1E5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin