Hep C cAg Antibody (C7-50): sc-57800

Hep C cAg Antibody (C7-50) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Hep C cAg protein of Hepatitis C Virus origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Hep C cAg monoclonal antibody (C7-50) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Hepatitis C virus, a small, enveloped, single-stranded, positive-sense RNA virus belonging to the Flaviviridae family, primarily replicates within hepatocytes in the liver, where Hepatitis C virus binds to specific receptors on the cell surface to gain entry. This localization is crucial as Hepatitis C virus exploits the liver′s cellular machinery for replication, leading to the production of approximately one trillion viral particles daily in infected individuals. The high mutation rate of Hepatitis C, resulting from the lack of proofreading capability in RNA polymerase, enables Hepatitis C virus to evade the host′s immune response, contributing to chronic infections that can progress to liver cirrhosis and hepatocellular carcinoma. Hep C cAg is highly conserved across different viral genotypes and plays a significant role in hepatic fibrogenesis by up-regulating connective tissue growth factor and transforming growth factor beta 1, highlighting Hep C cAg′s importance in the pathology of Hepatitis C infection.

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Hep C cAg Antibody (C7-50) References:

1. The roles of hepatitis C virus proteins in modulation of cellular functions: a novel action mechanism of the HCV core protein on gene regulation by nuclear hormone receptors.  |  Watashi, K. and Shimotohno, K. 2003. Cancer Sci. 94: 937-43. PMID: 14611668
2. Detection and quantitation of HCV core protein in single hepatocytes by means of laser capture microdissection and enzyme-linked immunosorbent assay.  |  Sansonno, D., et al. 2004. J Viral Hepat. 11: 27-32. PMID: 14738555
3. Nucleic acid binding properties and intermediates of HCV core protein multimerization in Pichia pastoris.  |  Acosta-Rivero, N., et al. 2004. Biochem Biophys Res Commun. 323: 926-31. PMID: 15381089
4. Morphological alteration and biological properties of hepatocytes not related to tumorigenesis following transfection with HCV core protein.  |  Gu, J., et al. 2005. J Viral Hepat. 12: 20-6. PMID: 15655044
5. Profiles of HCV core protein and viremia in chronic hepatitis C: possible protective role of core antigen in liver damage.  |  Carabaich, A., et al. 2005. J Med Virol. 76: 55-60. PMID: 15778969
6. HCV core protein augments cyclosporine immunosuppression.  |  Kimball, P., et al. 2005. Transplant Proc. 37: 652-3. PMID: 15848490
7. Thr 446 phosphorylation of PKR by HCV core protein deregulates G2/M phase in HCC cells.  |  Alisi, A., et al. 2005. J Cell Physiol. 205: 25-31. PMID: 15880455
8. HCV core protein promotes liver fibrogenesis via up-regulation of CTGF with TGF-beta1.  |  Shin, JY., et al. 2005. Exp Mol Med. 37: 138-45. PMID: 15886528
9. Hepatitis C virus (HCV) core protein enhances the immunogenicity of a co-delivered DNA vaccine encoding HCV structural antigens in mice.  |  Alvarez-Lajonchere, L., et al. 2006. Biotechnol Appl Biochem. 44: 9-17. PMID: 16367740
10. HCV core protein promotes hepatocyte proliferation and chemoresistance by inhibiting NR4A1.  |  Tan, Y. and Li, Y. 2015. Biochem Biophys Res Commun. 466: 592-8. PMID: 26392314
11. HCV Core protein represses DKK3 expression via epigenetic silencing and activates the Wnt/β-catenin signaling pathway during the progression of HCC.  |  Wang, X., et al. 2022. Clin Transl Oncol. 24: 1998-2009. PMID: 35768685
12. HCV Core Protein-ISX Axis Promotes Chronic Liver Disease Progression via Metabolic Remodeling and Immune Suppression.  |  Wang, LT., et al. 2023. Adv Sci (Weinh). 10: e2300644. PMID: 37316966

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Hep C cAg Antibody (C7-50) has 52 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Hep C cAg Antibody (C7-50) Specifications

Alternate Product Name: Hepatitis C virus core antigen

Product Clone Name: C7-50

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 21-40

Epitope Target: Hep C cAg

Epitope Species: Hepatitis C virus

Product Reactivity/Detection: the core antigen of Hep C transfected human and primate cell lines

Species Reactivity/Detection: Hepatitis C Virus

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 52 citations in various scientific publications

Available Conjugates/Forms for Hep C cAg Antibody (C7-50): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin