Hemoglobin α1 CRISPR/Cas9 KO Plasmid (m): sc-420800
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hemoglobin α1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Hemoglobin α1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hemoglobin α1 CRISPR/Cas9 KO Plasmid (m) | sc-420800 | 20 µg | $397.00 |
Overview
Hba-a1 encodes the mouse hemoglobin α1 subunit, a core component of the adult hemoglobin tetramer that binds heme and supports reversible oxygen transport in erythrocytes. Its expression is tightly coordinated during erythropoiesis with β-globin genes and heme biosynthetic enzymes, linking Hba-a1 to globin gene regulation, iron handling, and red blood cell maturation. Disruption of α-globin dosage perturbs hemoglobin assembly and redox balance, promoting ineffective erythropoiesis and hemolytic stress phenotypes that model features of α-thalassemia and related anemia biology. As a highly expressed, lineage-restricted gene, Hba-a1 is commonly used to interrogate regulatory architecture at the α-globin locus and mechanisms governing erythroid differentiation.
Hemoglobin α1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hba-a1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hba-a1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hba-a1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Hemoglobin α1 protein expression.
This CRISPR knockout system enables efficient generation of Hba-a1-deficient cell models for investigation of Hemoglobin α1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hba-a1 exon(s) critical for Hemoglobin α1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hba-a1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Hemoglobin α1 CRISPR/Cas9 KO Plasmid (m) and Hemoglobin α1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hba-a1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Hemoglobin α1 HDR Plasmid (m) and Hemoglobin α1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hba-a1 homology arms to support homology-directed repair at defined Hba-a1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.