hemicentin-1 CRISPR/Cas9 KO Plasmid (h): sc-405850
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hemicentin-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the hemicentin-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hemicentin-1 CRISPR/Cas9 KO Plasmid (h) | sc-405850 | 20 µg | $397.00 |
Overview
HMCN1 encodes hemicentin-1, a large extracellular matrix glycoprotein that helps organize basement membrane architecture and supports cell–matrix adhesion in tissues subject to mechanical stress. Hemicentin-1 participates in extracellular matrix assembly and remodeling, influencing processes such as epithelial integrity, tissue morphogenesis, and wound-related matrix dynamics. Disruption or dysregulation of HMCN1 has been linked to connective tissue and ocular phenotypes, and genetic studies have associated variants with age-related macular degeneration risk, highlighting relevance to retinal extracellular matrix biology. As an ECM component, hemicentin-1 provides a useful entry point for studying how matrix organization modulates cell migration, mechanotransduction, and barrier function.
hemicentin-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HMCN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HMCN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HMCN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish hemicentin-1 protein expression.
This CRISPR knockout system enables efficient generation of HMCN1-deficient cell models for investigation of hemicentin-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HMCN1 exon(s) critical for hemicentin-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HMCN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by hemicentin-1 CRISPR/Cas9 KO Plasmid (h) and hemicentin-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HMCN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by hemicentin-1 HDR Plasmid (h) and hemicentin-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HMCN1 homology arms to support homology-directed repair at defined HMCN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.