Heme Oxygenase 2/3 Antibody (A-3): sc-166342

Heme Oxygenase 2/3 Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody designed to target amino acids 1-300 at the N-terminus of mouse Heme Oxygenase 2, providing exceptional specificity and affinity for both Heme Oxygenase 2 and Heme Oxygenase 3 proteins. Anti-Heme Oxygenase 2/3 antibody (A-3) demonstrates broad species reactivity, effectively recognizing Heme Oxygenase 2 from mouse, rat, and human origins, as well as Heme Oxygenase 3 from mouse origin, making this an ideal choice for a variety of applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Heme oxygenases are essential microsomal enzymes predominantly localized in the endoplasmic reticulum, where these enzymes catalyze the degradation of heme into biliverdin, free iron, and carbon monoxide (CO), thereby playing a crucial role in cellular antioxidant defense and maintaining heme homeostasis. Heme Oxygenase 2, also called Hmox, HO2, and HO-2, is constitutively expressed across various mammalian tissues, with significant localization in the brain where this enzyme facilitates the continuous production of CO, a gaseous neurotransmitter that modulates neuronal signaling, vascular tone, and cellular responses to oxidative stress. The precise cellular localization of Heme Oxygenase 2 within key organelles such as the endoplasmic reticulum is vital for enzymatic function in heme catabolism and the localized generation of CO, which is integral to physiological processes including neurotransmission and vascular regulation. Heme Oxygenase 3 is present in a variety of tissues including the spleen, liver, thymus, prostate, heart, kidney, brain, and testis, and contains two heme regulatory motifs that may enhance heme-binding capacity and modulate enzymatic activity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Heme Oxygenase 2/3 Antibody (A-3) References:

1. Developmental biology of heme oxygenase.  |  Rodgers, PA. and Stevenson, DK. 1990. Clin Perinatol. 17: 275-91. PMID: 2196131
2. Heme oxygenase: function, multiplicity, regulatory mechanisms, and clinical applications.  |  Maines, MD. 1988. FASEB J. 2: 2557-68. PMID: 3290025
3. Isolation and characterization of the mouse heme oxygenase-1 gene. Distal 5' sequences are required for induction by heme or heavy metals.  |  Alam, J., et al. 1994. J Biol Chem. 269: 1001-9. PMID: 8288554
4. The heme oxygenase system: a regulator of second messenger gases.  |  Maines, MD. 1997. Annu Rev Pharmacol Toxicol. 37: 517-54. PMID: 9131263
5. Isolation and characterization of a cDNA from the rat brain that encodes hemoprotein heme oxygenase-3.  |  McCoubrey, WK., et al. 1997. Eur J Biochem. 247: 725-32. PMID: 9266719
6. Nitric oxide and carbon monoxide: parallel roles as neural messengers.  |  Snyder, SH., et al. 1998. Brain Res Brain Res Rev. 26: 167-75. PMID: 9651518
7. Heme oxygenase-1-derived carbon monoxide contributes to the suppression of acute hypertensive responses in vivo.  |  Motterlini, R., et al. 1998. Circ Res. 83: 568-77. PMID: 9734480

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Heme Oxygenase 2/3 Antibody (A-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Heme Oxygenase 2/3 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Heme Oxygenase 2/3 Antibody (A-3) Specifications

Alternate Product Name: Heme Oxygenase 2/3

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: Heme Oxygenase 2

Epitope Species: mouse

Product Reactivity/Detection: Heme Oxygenase 2 of mouse, rat and human origin, and Heme Oxygenase 3 of mouse origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Heme Oxygenase 2/3 Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Heme Oxygenase 1/HMOX1 antibody is Heme Oxygenase 1/HMOX1 Antibody (A-3): sc-136960.