HECTD1 CRISPR/Cas9 KO Plasmid (m): sc-431500
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HECTD1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HECTD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HECTD1 CRISPR/Cas9 KO Plasmid (m) | sc-431500 | 20 µg | $397.00 |
Overview
Hectd1 encodes HECTD1, a HECT-type E3 ubiquitin ligase that catalyzes ubiquitin transfer to substrate proteins to regulate their stability and signaling output. In mouse cells, HECTD1 has been linked to control of protein turnover and stress-responsive pathways, influencing cytoskeletal organization, cell polarity, and processes that shape tissue morphogenesis and developmental patterning. By modulating ubiquitin-dependent signaling nodes, HECTD1 can impact pathways governing proliferation, differentiation, and apoptosis. Dysregulation of ubiquitin ligase activity is broadly relevant to disease mechanisms involving proteostasis imbalance, aberrant signaling, and developmental defects, making Hectd1 a useful target for mechanistic studies in mammalian systems.
HECTD1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hectd1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hectd1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hectd1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HECTD1 protein expression.
This CRISPR knockout system enables efficient generation of Hectd1-deficient cell models for investigation of HECTD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hectd1 exon(s) critical for HECTD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hectd1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HECTD1 CRISPR/Cas9 KO Plasmid (m) and HECTD1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hectd1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HECTD1 HDR Plasmid (m) and HECTD1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hectd1 homology arms to support homology-directed repair at defined Hectd1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.