HECTD1 CRISPR/Cas9 KO Plasmid (h): sc-409864
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HECTD1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HECTD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HECTD1 Antibody (1E10): sc-517169
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HECTD1 CRISPR/Cas9 KO Plasmid (h) | sc-409864 | 20 µg | $397.00 |
Overview
HECTD1 encodes a HECT-type E3 ubiquitin ligase that mediates substrate ubiquitination to regulate proteostasis, protein turnover, and signaling amplitude in multiple cellular contexts. By controlling ubiquitin-dependent trafficking and degradation of pathway components, HECTD1 can influence processes such as cell-cycle progression, stress responses, and cytoskeletal dynamics that depend on timely remodeling of protein networks. Dysregulated E3 ligase activity is frequently linked to altered signaling fidelity and aberrant cellular phenotypes, making HECTD1 a relevant node for studying ubiquitin–proteasome system function in human cells. Research on HECTD1 supports mechanistic investigation of how ubiquitination interfaces with developmentally important and disease-associated signaling programs.
HECTD1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HECTD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HECTD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HECTD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HECTD1 protein expression.
This CRISPR knockout system enables efficient generation of HECTD1-deficient cell models for investigation of HECTD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HECTD1 exon(s) critical for HECTD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HECTD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HECTD1 CRISPR/Cas9 KO Plasmid (h) and HECTD1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HECTD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HECTD1 HDR Plasmid (h) and HECTD1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HECTD1 homology arms to support homology-directed repair at defined HECTD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.