Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Headpin CRISPR/Cas9 KO Plasmid (h): sc-409662
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Headpin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Headpin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Headpin Antibody (E-1): sc-398857
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Headpin CRISPR/Cas9 KO Plasmid (h) | sc-409662 | 20 µg | $397.00 |
Overview
SERPINB13 encodes the human serine protease inhibitor Headpin, a clade B (intracellular) serpin expressed prominently in stratified epithelia. Headpin regulates proteolysis by inhibiting select trypsin-like proteases, helping maintain epithelial barrier integrity and protease–antiprotease homeostasis during differentiation and tissue remodeling. By constraining aberrant protease activity, SERPINB13 can influence inflammatory signaling, cell survival, and stress responses associated with epithelial injury. Altered SERPINB13 expression has been reported across epithelial pathologies, making it a useful target for investigating mechanisms that couple protease control to keratinocyte biology and disease-relevant phenotypes.
Headpin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SERPINB13 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SERPINB13 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SERPINB13 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Headpin protein expression.
This CRISPR knockout system enables efficient generation of SERPINB13-deficient cell models for investigation of Headpin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SERPINB13 exon(s) critical for Headpin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SERPINB13 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Headpin CRISPR/Cas9 KO Plasmid (h) and Headpin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SERPINB13 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Headpin HDR Plasmid (h) and Headpin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SERPINB13 homology arms to support homology-directed repair at defined SERPINB13 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.