HE4 Double Nickase Plasmid (h): sc-402876-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HE4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HE4 Double Nickase Plasmid (h) and HE4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WFDC2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HE4 Antibody (3F9): sc-293473
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HE4 Double Nickase Plasmid (h) | sc-402876-NIC | 20 µg | $410.00 | |||
HE4 Double Nickase Plasmid (h2) | sc-402876-NIC-2 | 20 µg | $410.00 |
WFDC2 encodes human HE4, a secreted whey acidic protein domain-containing glycoprotein implicated in epithelial homeostasis and remodeling. HE4 has been linked to regulation of extracellular protease activity and matrix interactions, supporting processes such as cell adhesion, migration, and tissue organization. Altered WFDC2/HE4 expression is frequently studied in the context of tumor-associated signaling programs, including pathways influencing epithelial–mesenchymal plasticity and inflammatory microenvironment responses. As a measurable secreted factor, HE4 is also used as a molecular readout for investigating transcriptional regulation, secretion dynamics, and extracellular pathway perturbations in human cell models.
HE4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WFDC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WFDC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WFDC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WFDC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.