Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HDGF Double Nickase Plasmid (h): sc-402859-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HDGF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HDGF Double Nickase Plasmid (h) and HDGF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HDGF. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HDGF Antibody (E-7): sc-271344
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HDGF Double Nickase Plasmid (h) | sc-402859-NIC | 20 µg | $410.00 | |||
HDGF Double Nickase Plasmid (h2) | sc-402859-NIC-2 | 20 µg | $410.00 |
Hepatoma-derived growth factor (HDGF) is a heparin-binding nuclear protein that functions as a mitogenic and transcriptional regulator, linking extracellular cues to programs controlling cell-cycle progression, survival, and differentiation. HDGF participates in chromatin-associated processes and has been connected to signaling networks such as MAPK/ERK and PI3K/AKT that shape proliferation and stress responses. Dysregulated HDGF expression is reported across multiple tumor contexts and is frequently studied in relation to invasion, angiogenesis-associated gene expression, and resistance to cellular stress. In addition, HDGF has roles in development and tissue remodeling, making it relevant for investigating growth control mechanisms in human cell models.
HDGF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HDGF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HDGF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HDGF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HDGF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.