HCN4 Antibody (SHG 1E5): sc-58622

HCN4 Antibody (SHG 1E5) is a rat monoclonal IgG1 antibody that detects HCN4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-HCN4 antibody (SHG 1E5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HCN4 is a member of the hyperpolarization-activated cyclic nucleotide-binding channel family, which plays a crucial role in generating rhythmic electrical activity in excitable tissues such as the heart and brain. Specifically, HCN4 channels are predominantly expressed in the sinoatrial node of the heart, where these channels contribute to the pacemaker potential that regulates heart rate. This function is vital for maintaining proper cardiac rhythm and overall cardiovascular health. HCN4 is characterized by a core transmembrane segment domain and a C-terminal cyclic nucleotide-binding domain, which allows HCN4 to respond to changes in intracellular cyclic nucleotide levels, thus modulating activity in response to physiological signals. The genes encoding HCN channels are located on different chromosomes, with HCN4 mapping to chromosome 15q24-q25, highlighting the genetic diversity and regulatory mechanisms governing these important ion channels.

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HCN4 Antibody (SHG 1E5) References:

1. Two pacemaker channels from human heart with profoundly different activation kinetics.  |  Ludwig, A., et al. 1999. EMBO J. 18: 2323-9. PMID: 10228147
2. Molecular characterization of a slowly gating human hyperpolarization-activated channel predominantly expressed in thalamus, heart, and testis.  |  Seifert, R., et al. 1999. Proc Natl Acad Sci U S A. 96: 9391-6. PMID: 10430953
3. The human gene coding for HCN2, a pacemaker channel of the heart.  |  Vaccari, T., et al. 1999. Biochim Biophys Acta. 1446: 419-25. PMID: 10524219
4. Molecular mechanism of cAMP modulation of HCN pacemaker channels.  |  Wainger, BJ., et al. 2001. Nature. 411: 805-10. PMID: 11459060
5. Molecular basis for the different activation kinetics of the pacemaker channels HCN2 and HCN4.  |  Stieber, J., et al. 2003. J Biol Chem. 278: 33672-80. PMID: 12813043
6. The hyperpolarization-activated channel HCN4 is required for the generation of pacemaker action potentials in the embryonic heart.  |  Stieber, J., et al. 2003. Proc Natl Acad Sci U S A. 100: 15235-40. PMID: 14657344
7. Functional characterization of a trafficking-defective HCN4 mutation, D553N, associated with cardiac arrhythmia.  |  Ueda, K., et al. 2004. J Biol Chem. 279: 27194-8. PMID: 15123648
8. Pacemaker activity of the human sinoatrial node: effects of HCN4 mutations on the hyperpolarization-activated current.  |  Verkerk, AO. and Wilders, R. 2014. Europace. 16: 384-95. PMID: 24569893
9. HCN4 Gene Variations in Sudden Unexplained Nocturnal Death Syndrome in the Southern Han Chinese Population.  |  Wu, Q., et al. 2019. J Forensic Sci. 64: 1112-1118. PMID: 30452770
10. HCN4 knockdown in dorsal hippocampus promotes anxiety-like behavior in mice.  |  Günther, A., et al. 2019. Genes Brain Behav. 18: e12550. PMID: 30585408
11. Ectopic HCN4 expression drives mTOR-dependent epilepsy in mice.  |  Hsieh, LS., et al. 2020. Sci Transl Med. 12: PMID: 33208499
12. HCN4 current during human sinoatrial node-like action potentials.  |  Hoekstra, M., et al. 2021. Prog Biophys Mol Biol. 166: 105-118. PMID: 34153331

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HCN4 Antibody (SHG 1E5) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-HCN4 Antibody (SHG 1E5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HCN4 Antibody (SHG 1E5) Specifications

Alternate Product Name: HCN4

Product Clone Name: SHG 1E5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 1048-1085

Epitope Target: HCN4

Epitope Species: human

Product Reactivity/Detection: HCN4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for HCN4 Antibody (SHG 1E5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin