HCF2 Antibody (D-12) recommended for detection of HCF2 of mouse, rat and human origin by WB, IP, IF and ELISA

HCF2 Antibody (D-12): sc-393251

HCF2 Antibody (D-12) is rated 5.0 out of 5 by 1.
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Datasheets

    • HCF2 Antibody (D-12) is a mouse monoclonal mouse IgG2b κ (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 555-792 mapping at the C-terminus of HCF2 of human origin
    • recommended for detection of HCF2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How much sample do you recommend loading on the gel for Western blot detection using HCF2 (D-12): sc-393251?

Asked by: DefinitelyNotMatt
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Produced positive Western blot data of HCF2 Produced positive Western blot data of HCF2 expression in Jurkat, Hs 181 Tes, RT-4 and U-251-MG whole cell lysates and human testis tissue extract . -SCBT QC
Date published: 2014-12-02
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