CD44 Antibody (F-4): sc-9960

HCAM Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects HCAM in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). HCAM (F-4) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. HCAM, also known as CD44, is a 742 amino acid single-pass type I membrane protein that plays a crucial role in cell adhesion and migration, functioning as a receptor for hyaluronic acid (HA) and interacting with ligands like osteopontin (OPN). This interaction is vital for mediating cell-cell and cell-matrix connections, which are essential during processes such as hematopoiesis, lymphocyte activation, and tumor metastasis. HCAM′s structure includes a Link domain and is subject to alternative splicing, resulting in multiple isoforms, including CD44R, CDw44, CD44S, CD44H (hematopoietic), and CD44E (epithelial). CD44H isoform shows high expression in cancer tissues, indicating significant involvement in tumor progression and highlighting HCAM′s importance in both normal physiological processes and pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD44 Antibody (F-4) References:

1. Novel hydrazino-carbonyl-amino-methylated polystyrene (HCAM) resin methodology for the synthesis of P1-aldehyde protease inhibitor candidates.  |  Siev, DV. and Semple, JE. 2000. Org Lett. 2: 19-22. PMID: 10814235
2. Fluticasone and salmeterol downregulate in vitro, fibroblast proliferation and ICAM-1 or H-CAM expression.  |  Silvestri, M., et al. 2001. Eur Respir J. 18: 139-45. PMID: 11510784
3. The adhesion molecules NCAM, HCAM, PECAM-1 and ICAM-1 in normal salivary gland tissues and salivary gland malignancies.  |  Perschbacher, K., et al. 2004. J Oral Pathol Med. 33: 230-6. PMID: 15061711
4. Immunohistochemical detection of the expression of the cell adhesion molecules E-cadherin, desmoglein-2, beta4-integrin, ICAM-1 and HCAM (CD44s) in Warthin's tumour of the parotid gland.  |  Andreadis, D., et al. 2005. Oral Oncol. 41: 799-805. PMID: 16043382
5. Hematopoietic progenitor cell expression of the H-CAM (CD44) homing-associated adhesion molecule.  |  Lewinsohn, DM., et al. 1990. Blood. 75: 589-95. PMID: 1688719
6. The hyaluronate receptor is a member of the CD44 (H-CAM) family of cell surface glycoproteins.  |  Culty, M., et al. 1990. J Cell Biol. 111: 2765-74. PMID: 1703543
7. Expression of beta 1-integrins, H-CAM (CD44) and LECAM-1 in primary gastro-intestinal B-cell lymphomas as compared to the adhesion receptor profile of the gut-associated lymphoid system, tonsil and peripheral lymph node.  |  Möller, P., et al. 1991. Int J Cancer. 49: 846-55. PMID: 1720425
8. Neoplastic human tissue mast cells express the adhesion molecule CD44/HCAM.  |  Horny, HP., et al. 1996. Virchows Arch. 429: 91-4. PMID: 8917709
9. Flow cytometric detection of CD44 (H-CAM) in hairy cell leukemia.  |  Rutella, S., et al. 1996. Leuk Lymphoma. 21: 497-500. PMID: 9172816
10. Expression of CD44 (HCAM) in small lymphocytic and mantle cell lymphoma.  |  Aguilera, NS., et al. 1998. Hum Pathol. 29: 1134-9. PMID: 9781654

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD44 Antibody (F-4) has 57 citations in a variety of scientific publications.

Primary antibodies like Anti-CD44 Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD44 Antibody (F-4) Specifications

Alternate Product Name: CD44

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 21-320

Epitope Target: HCAM

Epitope Species: human

Product Reactivity/Detection: HCAM

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 57 citations in various scientific publications

Available Conjugates/Forms for CD44 Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD44 antibody is CD44 Antibody (DF1485): sc-7297.