Date published: 2025-9-24

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CD44 Antibody (F-4): sc-9960

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Datasheets
  • CD44 Antibody (F-4) is a mouse monoclonal IgG1 κ, cited in 57 publications, provided at 200 µg/ml
  • raised against amino acids 21-320 of HCAM of human origin
  • Anti-CD44 Antibody (F-4) is recommended for detection of HCAM of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
  • Anti-CD44 Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD44 (F-4): sc-9960.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CD44 Antibody (F-4) for WB and IHC(P) applications. These reagents are now offered in bundles with CD44 Antibody (F-4) (see ordering information below).

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HCAM Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects HCAM in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). HCAM (F-4) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. HCAM, also known as CD44, is a 742 amino acid single-pass type I membrane protein that plays a crucial role in cell adhesion and migration, functioning as a receptor for hyaluronic acid (HA) and interacting with ligands like osteopontin (OPN). This interaction is vital for mediating cell-cell and cell-matrix connections, which are essential during processes such as hematopoiesis, lymphocyte activation, and tumor metastasis. HCAM′s structure includes a Link domain and is subject to alternative splicing, resulting in multiple isoforms, including CD44R, CDw44, CD44S, CD44H (hematopoietic), and CD44E (epithelial). CD44H isoform shows high expression in cancer tissues, indicating significant involvement in tumor progression and highlighting HCAM′s importance in both normal physiological processes and pathological conditions.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

CD44 Antibody (F-4)

sc-9960
200 µg/ml
$316.00

CD44 Antibody (F-4): m-IgG Fc BP-HRP Bundle

sc-526527
200 µg Ab; 10 µg BP
$354.00

CD44 Antibody (F-4): m-IgG1 BP-HRP Bundle

sc-531900
200 µg Ab; 20 µg BP
$354.00

CD44 Antibody (F-4) AC

sc-9960 AC
500 µg/ml, 25% agarose
$416.00

CD44 Antibody (F-4) HRP

sc-9960 HRP
200 µg/ml
$316.00

CD44 Antibody (F-4) FITC

sc-9960 FITC
200 µg/ml
$330.00

CD44 Antibody (F-4) PE

sc-9960 PE
200 µg/ml
$343.00

CD44 Antibody (F-4) Alexa Fluor® 488

sc-9960 AF488
200 µg/ml
$357.00

CD44 Antibody (F-4) Alexa Fluor® 546

sc-9960 AF546
200 µg/ml
$357.00

CD44 Antibody (F-4) Alexa Fluor® 594

sc-9960 AF594
200 µg/ml
$357.00

CD44 Antibody (F-4) Alexa Fluor® 647

sc-9960 AF647
200 µg/ml
$357.00

CD44 Antibody (F-4) Alexa Fluor® 680

sc-9960 AF680
200 µg/ml
$357.00

CD44 Antibody (F-4) Alexa Fluor® 790

sc-9960 AF790
200 µg/ml
$357.00

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Thank you for your question Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Santa Cruz TS
Date published: 2017-01-27
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Rated 5 out of 5 by from CD44 (HCAM) WBIt worked well in western blots of colorectal cancer cell lines. Clear band and clean background
Date published: 2024-06-07
Rated 4 out of 5 by from Excellent antibodyThis antibody is suitable for WB, IHC, IF, and flow cytometry assays.
Date published: 2023-03-14
Rated 2 out of 5 by from Strong background and very faint band in WBThere were very faint bands at the correct size and pretty strong backgorund in WB (1:500, 5% Milk-TBST, 50 µg CRC cell line protein lysate).
Date published: 2021-05-04
Rated 5 out of 5 by from Excellent antibodyThis antibody works great for Immunohistochemistry.
Date published: 2019-05-20
Rated 5 out of 5 by from great satisfiedI thank sending me for free trial. I am in my mind to order some antibody, if anything, for appreciation.
Date published: 2017-09-22
Rated 1 out of 5 by from no signalNo signal detected in Western Blot Analysis even when using the recommendated positive control.
Date published: 2017-08-04
Rated 5 out of 5 by from 产品很不错我之前用了很多家的抗体,一直做的效果都不好,用了贵公司的抗体,本来抱着试试的心态,没想到结果很好,希望贵公司一直推出这样好用的抗体。
Date published: 2017-06-23
Rated 5 out of 5 by from Reliable and Specific!With a broad range of species reactivity and application usage, SC-9960 suits all your HCAM detection needs. Having been featured in over 24 publications, this antibody is a proven winner.
Date published: 2016-12-20
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CD44 Antibody (F-4) is rated 4.5 out of 5 by 17.
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