HBXIP Double Nickase Plasmid (h): sc-405228-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HBXIP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HBXIP Double Nickase Plasmid (h) and HBXIP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LAMTOR5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HBXIP Antibody (H-5): sc-373980
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HBXIP Double Nickase Plasmid (h) | sc-405228-NIC | 20 µg | $410.00 | |||
HBXIP Double Nickase Plasmid (h2) | sc-405228-NIC-2 | 20 µg | $410.00 |
LAMTOR5 encodes HBXIP, a component of the Ragulator complex on late endosomes/lysosomes that supports amino acid–dependent activation of mTORC1 and coordinates nutrient sensing with cell growth and metabolism. HBXIP also participates in endosomal trafficking and has been reported to modulate transcriptional and cell-cycle–associated processes through protein–protein interactions. Through these roles, LAMTOR5/HBXIP links lysosomal signaling to proliferative and metabolic programs, making it relevant for studies of oncogenic signaling, stress adaptation, and altered nutrient utilization observed across multiple disease contexts. Its involvement in mTOR-related networks also connects it to autophagy regulation and lysosome-associated signaling dynamics.
HBXIP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LAMTOR5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LAMTOR5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LAMTOR5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LAMTOR5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.