HBP1 Antibody (G-8): sc-515281

HBP1 Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects HBP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HBP1 Antibody (G-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HMG-box protein-1 (HBP1) plays a crucial role in regulating cell differentiation and proliferation as a member of the high mobility group (HMG) family of transcription factors, which are characterized by a conserved HMG box that facilitates DNA binding. HBP1 is primarily located in the nucleus, where HBP1 interacts with key tumor suppressor proteins such as Rb and p130, initiating cell cycle arrest that is essential for terminal cell differentiation. This initial arrest is followed by the expression of tissue-specific genes, which are critical for the development of specialized cell types. HBP1′s continued activity can inhibit the expression of these genes by associating with MyoD proteins, which are vital for muscle cell differentiation. The balance between HBP1 activity and MyoD-induced gene transcription is therefore pivotal in determining the progression of terminal differentiation, highlighting HBP1′s importance in cellular development and potential implications in cancer biology and regenerative medicine.

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HBP1 Antibody (G-8) References:

1. Effects of overexpression of HBP1 upon growth and differentiation of leukemic myeloid cells.  |  Yao, CJ., et al. 2005. Leukemia. 19: 1958-68. PMID: 16179914
2. The HBP1 transcriptional repressor participates in RAS-induced premature senescence.  |  Zhang, X., et al. 2006. Mol Cell Biol. 26: 8252-66. PMID: 16966377
3. Alterations of the HBP1 transcriptional repressor are associated with invasive breast cancer.  |  Paulson, KE., et al. 2007. Cancer Res. 67: 6136-45. PMID: 17616670
4. The tumor suppressor protein HBP1 is a novel c-myc-binding protein that negatively regulates c-myc transcriptional activity.  |  Escamilla-Powers, JR., et al. 2010. J Biol Chem. 285: 4847-58. PMID: 20008325
5. HBP1-mediated transcriptional regulation of DNA methyltransferase 1 and its impact on cell senescence.  |  Pan, K., et al. 2013. Mol Cell Biol. 33: 887-903. PMID: 23249948
6. Transcription Factor HBP1 Enhances Radiosensitivity by Inducing Apoptosis in Prostate Cancer Cell Lines.  |  Chen, Y., et al. 2016. Anal Cell Pathol (Amst). 2016: 7015659. PMID: 26942107
7. HBP1-mediated Regulation of p21 Protein through the Mdm2/p53 and TCF4/EZH2 Pathways and Its Impact on Cell Senescence and Tumorigenesis.  |  Chen, Y., et al. 2016. J Biol Chem. 291: 12688-12705. PMID: 27129219
8. Transcription factor HBP1 is a direct anti-cancer target of transcription factor FOXO1 in invasive oral cancer.  |  Chan, CY., et al. 2017. Oncotarget. 8: 14537-14548. PMID: 28099936
9. Transcription factor HMG box-containing protein 1 (HBP1) modulates mitotic clonal expansion (MCE) during adipocyte differentiation.  |  Chan, CY., et al. 2018. J Cell Physiol. 233: 4205-4215. PMID: 29030964
10. MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1.  |  Cao, Z., et al. 2019. Oncogene. 38: 4835-4855. PMID: 30816344

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HBP1 Antibody (G-8) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-HBP1 Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HBP1 Antibody (G-8) Specifications

Alternate Product Name: HBP1

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 477-502

Epitope Target: HBP1

Epitope Species: human

Product Reactivity/Detection: HBP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for HBP1 Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent