Hbb-y CRISPR/Cas9 KO Plasmid (m): sc-420805
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hbb-y CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Hbb-y genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hbb-y CRISPR/Cas9 KO Plasmid (m) | sc-420805 | 20 µg | $397.00 |
Overview
Hbb-y encodes a mouse hemoglobin beta-like subunit that contributes to hemoglobin tetramer assembly and oxygen transport in erythrocytes. As part of the erythroid differentiation program, Hbb-y expression is coordinated with heme biosynthesis, globin chain balance, and red blood cell maturation pathways regulated by erythroid transcriptional networks. Alterations in beta-globin–like genes can perturb hemoglobin stability and red cell physiology, providing models for studying mechanisms relevant to anemia, hemoglobinopathies, and stress erythropoiesis. In mice, Hbb-y is also useful for dissecting developmental and strain-specific regulation within the beta-globin gene cluster.
Hbb-y CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hbb-y gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hbb-y together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hbb-y open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Hbb-y protein expression.
This CRISPR knockout system enables efficient generation of Hbb-y-deficient cell models for investigation of Hbb-y signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hbb-y exon(s) critical for Hbb-y function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hbb-y genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Hbb-y CRISPR/Cas9 KO Plasmid (m) and Hbb-y CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hbb-y locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Hbb-y HDR Plasmid (m) and Hbb-y HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hbb-y homology arms to support homology-directed repair at defined Hbb-y target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.