Hbb-bh1 CRISPR/Cas9 KO Plasmid (m): sc-420804
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hbb-bh1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Hbb-bh1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hbb-bh1 CRISPR/Cas9 KO Plasmid (m) | sc-420804 | 20 µg | $397.00 |
Overview
Hbb-bh1 encodes an embryonic β-like globin subunit that contributes to hemoglobin assembly and oxygen transport during early mouse development. Its expression is developmentally regulated within the β-globin gene cluster and coordinated with erythroid differentiation programs controlled by transcriptional and epigenetic regulators. Hbb-bh1 participates in heme–globin balance and redox homeostasis in primitive erythrocytes, linking it to pathways that influence erythrocyte maturation and oxidative stress responses. Dysregulation of globin gene switching and hemoglobin composition is relevant to modeling hemoglobinopathies and studying mechanisms that shape erythroid lineage specification.
Hbb-bh1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hbb-bh1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hbb-bh1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hbb-bh1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Hbb-bh1 protein expression.
This CRISPR knockout system enables efficient generation of Hbb-bh1-deficient cell models for investigation of Hbb-bh1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hbb-bh1 exon(s) critical for Hbb-bh1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hbb-bh1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Hbb-bh1 CRISPR/Cas9 KO Plasmid (m) and Hbb-bh1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hbb-bh1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Hbb-bh1 HDR Plasmid (m) and Hbb-bh1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hbb-bh1 homology arms to support homology-directed repair at defined Hbb-bh1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.