Hbb-b2 CRISPR/Cas9 KO Plasmid (m): sc-420803
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hbb-b2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Hbb-b2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hbb-b2 CRISPR/Cas9 KO Plasmid (m) | sc-420803 | 20 µg | $397.00 |
Overview
Mouse Hbb-b2 encodes a beta-globin subunit that assembles with alpha-globin to form hemoglobin, the erythrocyte oxygen carrier essential for systemic oxygen delivery and carbon dioxide transport. Its expression is tightly coordinated during erythropoiesis through erythroid transcriptional programs and globin locus regulation, supporting red blood cell maturation and hemoglobin tetramer stability. Variation in beta-globin dosage or sequence can perturb hemoglobin function and red cell physiology, making Hbb-b2 a useful model gene for studying hemoglobinopathies and anemia-related mechanisms. As part of the globin network, Hbb-b2 provides a tractable readout for pathways controlling erythroid differentiation, oxidative stress handling, and hypoxia-responsive biology.
Hbb-b2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hbb-b2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hbb-b2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hbb-b2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Hbb-b2 protein expression.
This CRISPR knockout system enables efficient generation of Hbb-b2-deficient cell models for investigation of Hbb-b2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hbb-b2 exon(s) critical for Hbb-b2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hbb-b2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Hbb-b2 CRISPR/Cas9 KO Plasmid (m) and Hbb-b2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hbb-b2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Hbb-b2 HDR Plasmid (m) and Hbb-b2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hbb-b2 homology arms to support homology-directed repair at defined Hbb-b2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.