HB9 Antibody (F-5): sc-515769

HB9 Antibody (F-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects HB9 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HB9 (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HB9, a homeobox transcription factor, plays a critical role in regulating gene expression during embryonic development and in specific adult tissues, particularly in motor neuron differentiation. HB9 function is essential for motor neuron column formation; in HB9-deficient mice, these columns are disorganized, leading to significant nerve defects such as absent phrenic and abducens nerves. Mutations in HB9 are associated with Curriano syndrome, presenting a triad of developmental anomalies including presacral tumor, sacral agenesis, and anorectal malformation. In human bone marrow, HB9 expression correlates with CD34 expression, with increased levels in CD34+ cells treated with interleukin-3 and granulocyte macrophage-colony-stimulating factor, highlighting HB9′s importance in hematopoietic development. During early murine development, HB9 is expressed in pancreatic buds and later in differentiating beta cells within the islets of Langerhans, indicating a vital role in pancreatic development and function. HB9 absence leads to underdeveloped pancreatic structures, underscoring HB9′s significance in both embryonic and adult physiology.

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HB9 Antibody (F-5) References:

1. Selective agenesis of the dorsal pancreas in mice lacking homeobox gene Hlxb9.  |  Li, H., et al. 1999. Nat Genet. 23: 67-70. PMID: 10471501
2. Pancreas dorsal lobe agenesis and abnormal islets of Langerhans in Hlxb9-deficient mice.  |  Harrison, KA., et al. 1999. Nat Genet. 23: 71-5. PMID: 10471502
3. Requirement for the homeobox gene Hb9 in the consolidation of motor neuron identity.  |  Arber, S., et al. 1999. Neuron. 23: 659-74. PMID: 10482234
4. Active suppression of interneuron programs within developing motor neurons revealed by analysis of homeodomain factor HB9.  |  Thaler, J., et al. 1999. Neuron. 23: 675-87. PMID: 10482235
5. Spectrum of mutations and genotype-phenotype analysis in Currarino syndrome.  |  Köchling, J., et al. 2001. Eur J Hum Genet. 9: 599-605. PMID: 11528505
6. Two diverged human homeobox genes involved in the differentiation of human hematopoietic progenitors map to chromosome 1, bands q41-42.1.  |  Najfeld, V., et al. 1992. Genes Chromosomes Cancer. 5: 343-7. PMID: 1283323
7. Selective expression of two homeobox genes in CD34-positive cells from human bone marrow.  |  Deguchi, Y. and Kehrl, JH. 1991. Blood. 78: 323-8. PMID: 1712647
8. Genome-wide identification of Drosophila Hb9 targets reveals a pivotal role in directing the transcriptome within eight neuronal lineages, including activation of nitric oxide synthase and Fd59a/Fox-D.  |  Lacin, H., et al. 2014. Dev Biol. 388: 117-33. PMID: 24512689
9. Pancreatic HB9 protein level is affected by early life stress in young adult rats: possible involvement of TNF-α and corticosterone.  |  Sadeghimahalli, F., et al. 2021. Arch Physiol Biochem. 127: 406-413. PMID: 31368362
10. Elimination of glutamatergic transmission from Hb9 interneurons does not impact treadmill locomotion.  |  Koronfel, LM., et al. 2021. Sci Rep. 11: 16008. PMID: 34362940
11. Transcription Factor Hb9 Is Expressed in Glial Cell Lineages in the Developing Mouse Spinal Cord.  |  Letchuman, S., et al. 2022. eNeuro. 9: PMID: 36265906
12. A homeobox gene, HLXB9, is the major locus for dominantly inherited sacral agenesis.  |  Ross, AJ., et al. 1998. Nat Genet. 20: 358-61. PMID: 9843207

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HB9 Antibody (F-5) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-HB9 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HB9 Antibody (F-5) Specifications

Alternate Product Name: HB9

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 179-203

Epitope Target: HB9

Epitope Species: human

Product Reactivity/Detection: HB9

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for HB9 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin