HB-EGF Antibody (H-1): sc-365182
- HB-EGF Antibody (H-1) is a mouse monoclonal IgG2a κ, cited in 10 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 183-213 within a C-terminal cytoplasmic domain of HB-EGF of mouse origin
- Anti-HB-EGF Antibody (H-1) is recommended for detection of precursor HB-EGF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-HB-EGF Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HB-EGF (H-1): sc-365182.
- m-IgGκ BP-HRP is the preferred secondary detection reagent for HB-EGF Antibody (H-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with HB-EGF Antibody (H-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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SEE ALSO...
HB-EGF Antibody (H-1) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect Heparin-binding EGF-like growth factor (HB-EGF), also known as HBEGF or the 87-amino acid EGF-like glycoprotein. HB-EGF monoclonal antibody (H-1) specifically binds to an epitope spanning amino acids 183-213 within the C-terminal cytoplasmic domain of HB-EGF from mouse origin, ensuring precise detection of precursor HB-EGF across multiple species, including mouse, rat, and human, in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). HB-EGF is a crucial member of the epidermal growth factor (EGF) family of mitogens, characterized by its dual structure comprising a membrane-bound precursor with a transmembrane domain and signal peptide, and a mature soluble form resulting from proteolytic cleavage. This structural versatility allows HB-EGF to engage in both autocrine and paracrine signaling, facilitating essential cellular processes such as proliferation, differentiation, and survival. The membrane-bound form of HB-EGF also serves as the receptor for diphtheria toxin, highlighting its significant role in cellular interactions and pathogen entry. The precise structural configuration of HB-EGF is vital for its interaction with the EGF receptor (EGFR) and heparin sulfate proteoglycans on the cell surface, which are critical for effective signal transduction and cellular communication. HB-EGF (H-1) antibody is available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing researchers with versatile options for diverse experimental needs. Anti-HB-EGF antibody (H-1) recognition of precursor forms of HB-EGF in multiple species makes this antibody an indispensable tool for studying growth factor signaling pathways, tissue regeneration, and disease mechanisms such as cancer and cardiovascular disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HB-EGF Antibody (H-1) | sc-365182 | 200 µg/ml | $316.00 | |||
HB-EGF Antibody (H-1): m-IgGκ BP-HRP Bundle | sc-522155 | 200 µg Ab, 40 µg BP | $354.00 | |||
HB-EGF Antibody (H-1) AC | sc-365182 AC | 500 µg/ml, 25% agarose | $416.00 | |||
HB-EGF Antibody (H-1) HRP | sc-365182 HRP | 200 µg/ml | $316.00 | |||
HB-EGF Antibody (H-1) FITC | sc-365182 FITC | 200 µg/ml | $330.00 | |||
HB-EGF Antibody (H-1) PE | sc-365182 PE | 200 µg/ml | $343.00 | |||
HB-EGF Antibody (H-1) Alexa Fluor® 488 | sc-365182 AF488 | 200 µg/ml | $357.00 | |||
HB-EGF Antibody (H-1) Alexa Fluor® 546 | sc-365182 AF546 | 200 µg/ml | $357.00 | |||
HB-EGF Antibody (H-1) Alexa Fluor® 594 | sc-365182 AF594 | 200 µg/ml | $357.00 | |||
HB-EGF Antibody (H-1) Alexa Fluor® 647 | sc-365182 AF647 | 200 µg/ml | $357.00 | |||
HB-EGF Antibody (H-1) Alexa Fluor® 680 | sc-365182 AF680 | 200 µg/ml | $357.00 | |||
HB-EGF Antibody (H-1) Alexa Fluor® 790 | sc-365182 AF790 | 200 µg/ml | $357.00 | |||
HB-EGF (H-1) Neutralizing Peptide | sc-365182 P | 100 µg/0.5 ml | $68.00 |