HB-EGF Antibody (G-11): sc-74441

HB-EGF Antibody (G-11) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect Heparin-binding EGF-like growth factor (HB-EGF), also known as HBEGF or the 87-amino acid EGF-like glycoprotein. HB-EGF monoclonal antibody (G-11) targets amino acids 121-208 of the mature human HB-EGF protein, ensuring precise binding and reliable detection in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) assays. HB-EGF is a pivotal member of the epidermal growth factor (EGF) family, functioning as a potent mitogen by binding to the EGF receptor (EGFR) and heparin sulfate proteoglycans on the cell surface, which is essential for regulating cell proliferation, differentiation, and survival. The HB-EGF gene encodes a 208 amino acid precursor that undergoes enzymatic cleavage to produce the active, soluble form of 86 amino acids, highlighting its critical role in cellular signaling processes. Additionally, the membrane-bound form of HB-EGF serves as the diphtheria toxin receptor, underscoring its importance in both physiological and pathological contexts. HB-EGF (G-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing versatility for a wide range of experimental applications. Anti-HB-EGF antibody (G-11) recognizes both precursor and mature forms of HB-EGF in human samples, making this antibody an invaluable tool for investigating growth factor signaling pathways and related disease mechanisms. HB-EGF (G-11) monoclonal antibody facilitates advanced research in molecular biology and therapeutic development.

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HB-EGF Antibody (G-11) References:

1. A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF.  |  Higashiyama, S., et al. 1991. Science. 251: 936-9. PMID: 1840698
2. Phorbol ester induces the rapid processing of cell surface heparin-binding EGF-like growth factor: conversion from juxtacrine to paracrine growth factor activity.  |  Goishi, K., et al. 1995. Mol Biol Cell. 6: 967-80. PMID: 7579712
3. Diphtheria toxin endocytosis and membrane translocation are dependent on the intact membrane-anchored receptor (HB-EGF precursor): studies on the cell-associated receptor cleaved by a metalloprotease in phorbol-ester-treated cells.  |  Lanzrein, M., et al. 1995. Biochem J. 310 (Pt 1): 285-9. PMID: 7646457
4. Membrane-anchored heparin-binding EGF-like growth factor (HB-EGF) and diphtheria toxin receptor-associated protein (DRAP27)/CD9 form a complex with integrin alpha 3 beta 1 at cell-cell contact sites.  |  Nakamura, K., et al. 1995. J Cell Biol. 129: 1691-705. PMID: 7790364
5. Diphtheria toxin binds to the epidermal growth factor (EGF)-like domain of human heparin-binding EGF-like growth factor/diphtheria toxin receptor and inhibits specifically its mitogenic activity.  |  Mitamura, T., et al. 1995. J Biol Chem. 270: 1015-9. PMID: 7836353
6. Increased expression of heparin binding epidermal growth-factor-like growth factor mRNA in the kidney of streptozotocin-induced diabetic rats.  |  Lee, YJ., et al. 1995. Biochem Biophys Res Commun. 207: 216-22. PMID: 7857268
7. The binding of HB-EGF to tumour cells is blocked by mAbs which act as EGF and TGF alpha antagonists.  |  Modjtahedi, H. and Dean, C. 1995. Biochem Biophys Res Commun. 207: 389-97. PMID: 7857293

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HB-EGF Antibody (G-11) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-HB-EGF Antibody (G-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HB-EGF Antibody (G-11) Specifications

Alternate Product Name: Heparin-binding epidermal growth factor (EGF)-like growth factor

Product Clone Name: G-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 121-208

Epitope Target: mature HB-EGF

Epitope Species: human

Product Reactivity/Detection: precursor and mature HB-EGF

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for HB-EGF Antibody (G-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin