Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HAUSP CRISPR/Cas9 KO Plasmid (m): sc-434244
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HAUSP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HAUSP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HAUSP Antibody (H-12): sc-137008
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAUSP CRISPR/Cas9 KO Plasmid (m) | sc-434244 | 20 µg | $397.00 |
Overview
Mouse Usp7 encodes HAUSP, a ubiquitin-specific protease that removes ubiquitin from key regulators of genome integrity and transcription, including the p53–MDM2 axis, thereby modulating protein stability and signaling dynamics. HAUSP participates in ubiquitin–proteasome pathway control, DNA damage response, replication stress handling, and chromatin-associated processes that influence cell-cycle progression and apoptosis. Through deubiquitination of factors involved in checkpoint signaling and repair, Usp7 helps shape outcomes following genotoxic stress and oncogene activation. Dysregulated USP7/HAUSP activity has been linked to altered tumor suppressor networks, immune and inflammatory signaling balance, and broader cancer-relevant phenotypes in experimental models.
HAUSP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Usp7 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Usp7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Usp7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HAUSP protein expression.
This CRISPR knockout system enables efficient generation of Usp7-deficient cell models for investigation of HAUSP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Usp7 exon(s) critical for HAUSP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Usp7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HAUSP CRISPR/Cas9 KO Plasmid (m) and HAUSP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Usp7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HAUSP HDR Plasmid (m) and HAUSP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Usp7 homology arms to support homology-directed repair at defined Usp7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.